2.7.1.175: maltokinase

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For detailed information about maltokinase, go to the full flat file.

Word Map on EC 2.7.1.175

2.7.1.175

trehalose

mycobacteria

actinoplanes

glycogen

smegmatis

missouriensis

glucan

aminoglycoside

tuberculosis

envelope

tetramer

drug development

synthesis

Reaction

Synonyms

ATP:maltose 1-phosphotransferase, Mak, Mak1, maltose-1-phosphate synthetase, Msm Pep2, Pep2

ECTree

2 Transferases

2.7 Transferring phosphorus-containing groups

2.7.1 Phosphotransferases with an alcohol group as acceptor

2.7.1.175 maltokinase

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Results	in table
1	Activating Compound
7177	AA Sequence
3	Application
1	CAS Registry Number
6	Cloned(Commentary)
12	Cofactor
2	Crystallization (Commentary)
30	General Information
1	General Stability
1	Inhibitors
8	KM Value [mM]
13	Metals/Ions
9	Molecular Weight [Da]
11	Natural Substrates/ Products (Substrates)
19	Organism
3	Pathway
15	PDB ID
10	pH Optimum
1	pH Range
1	pI Value
31	Protein Variants
7	Purification (Commentary)
3	Reaction
17	Reference
1	Specific Activity [micromol/min/mg]
1	Storage Stability
21	Substrates and Products (Substrate)
14	Subunits
18	Synonyms
1	Systematic Name
8	Temperature Optimum [°C]
2	Temperature Range [°C]

Purification

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Purification on EC 2.7.1.175 - maltokinase

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native enzyme by heat treatment at 50°C for 30 min, anion exchange chromatography, ammonium sulfate fractionation, hydrophobic interaction chromatography, ultra- and gel filtration, and another step of anion exchange chromatography

Actinoplanes missouriensis

717163

recombinant C-terminally His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity and anion exchange chromatography, followed by gel filtration

2 entries

recombinant enzyme from Escherichia coli by anion exchange chromatography and gel filtration

Mycobacterium tuberculosis variant bovis

717432

recombinant His-tagged enzyme from Mycobacterium smegmatis by nickel affinity chromatography

Mycobacterium tuberculosis

O07177

737320

recombinant His-tagged enzyme from Streptomyces lividans strain 66 by nickel affinity chromatography

2 entries