Information on EC 2.7.1.175 - maltokinase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.7.1.175
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RECOMMENDED NAME
GeneOntology No.
maltokinase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + maltose = ADP + alpha-maltose 1-phosphate
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Starch and sucrose metabolism
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Metabolic pathways
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SYSTEMATIC NAME
IUBMB Comments
ATP:alpha-maltose 1-phosphotransferase
Requires Mg2+ for activity.
CAS REGISTRY NUMBER
COMMENTARY hide
179987-43-0
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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Manually annotated by BRENDA team
gene mak
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Manually annotated by BRENDA team
gene pep2
UniProt
Manually annotated by BRENDA team
gene pep2
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
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genetic environment of the mak gene in different organisms. Organization of the region containing the mak gene, overview
metabolism
physiological function
maltokinase is the enzyme responsible for the ATP-dependent formation of maltose 1-phosphate
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + maltose
ADP + alpha-maltose 1-phosphate
show the reaction diagram
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product analysis and determination by chemical analysis and NMR spectroscopy
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?
ATP + maltose
ADP + maltose 1-phosphate
show the reaction diagram
GTP + maltose
GDP + maltose 1-phosphate
show the reaction diagram
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NMR spectroscopical product analysis
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?
UTP + maltose
UDP + maltose 1-phosphate
show the reaction diagram
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NMR spectroscopical product analysis
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?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + maltose
ADP + alpha-maltose 1-phosphate
show the reaction diagram
-
-
-
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?
ATP + maltose
ADP + maltose 1-phosphate
show the reaction diagram
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-
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-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
GTP
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can substitute for ATP
UTP
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can substitute for ATP
additional information
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CTP and TTP show no activity, while GTP with 2.1% activity compared to ATP and UTP with 2.7% show only poor activity as phosphoryl-group donors
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Co2+
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activates, 41% activity compared to Mg2+
Mn2+
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activates, 14% activity compared to Mg2+
NaCl
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addition of 50 mM NaCl markedly enhances Mak stability
additional information
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divalent cations are required for activity and Mg2+ is the best activator
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
glycerol
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strongly inhibits Mak activity, even at very low concentrations of 1%
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.54 - 0.74
ATP
2.52 - 2.6
maltose
additional information
additional information
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
144.8
purified His-tagged recombinant enzyme, pH 7.0, 45C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 9
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at 37C
7.75
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in Tris/HCl buffer
7.8 - 8
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assay at
8.5
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in TEA/HCl buffer
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 11
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activity range
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
20 - 65
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activity range
additional information
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the activity of maltokinase increases 2.5fold with a maximum at 55C, but decreases rapidly at temperatures above 60C
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.3
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isoelectric focusing
PDB
SCOP
CATH
ORGANISM
UNIPROT
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium vanbaalenii (strain DSM 7251 / PYR-1)
Mycobacterium vanbaalenii (strain DSM 7251 / PYR-1)
Mycobacterium vanbaalenii (strain DSM 7251 / PYR-1)
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
addition of 50 mM NaCl markedly enhances Mak stability
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4C, purified recombinant His-tagged enzyme, 7 days, about 40% of activity remaining, at pH 7.5, most of the activity is lost after freeze/thawing at -20C. 10 mM maltose only slightly improve the stability at 4C to about 50% of maximal activity, but addition of 50 mM NaCl dramatically improve the stability of the enzyme with over 90% activity remaining after 1 week at 4C
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
native enzyme by heat treatment at 50C for 30 min, anion exchange chromatography, ammonium sulfate fractionation, hydrophobic interaction chromatography, ultra- and gel filtration, and another step of anion exchange chromatography
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recombinant enzyme from Escherichia coli by anion exchange chromatography and gel filtration
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recombinant His-tagged enzyme from Streptomyces lividans strain 66 by nickel affinity chromatography
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
gene mak, DNA and amino acid sequence determination and analysis, high level overexpression of His-tagged Mak in Escherichia coli
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gene mak1, located in a gene cluster, DNA and amino acid sequence determination and analysis, sequence comparisons, expression of the His-tagged enzyme in Streptomyces lividans strain 66 using vector pMJP7
gene pep2, sequence comparisons, expression of the His-tagged enzyme in Streptomyces lividans strain 66 using vector pMJP7
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
drug development
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Mak may be a potential drug target in Mycobacterium tuberculosis
synthesis