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Literature summary for 2.7.1.175 extracted from

  • Niehues, B.; Jossek, R.; Kramer, U.; Koch, A.; Jarling, M.; Schroeder, W.; Pape, H.
    Isolation and characterization of maltokinase (ATP:maltose 1-phosphotransferase) from Actinoplanes missouriensis (2003), Arch. Microbiol., 180, 233-239.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Michaelis-Menten kinetics Actinoplanes missouriensis
0.54
-
ATP pH 8.5, 22°C Actinoplanes missouriensis
2.6
-
maltose pH 8.5, 22°C Actinoplanes missouriensis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Actinoplanes missouriensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57000
-
1 * 57000, SDS-PAGE Actinoplanes missouriensis

Organism

Organism UniProt Comment Textmining
Actinoplanes missouriensis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme by heat treatment at 50°C for 30 min, anion exchange chromatography, ammonium sulfate fractionation, hydrophobic interaction chromatography, ultra- and gel filtration, and another step of anion exchange chromatography Actinoplanes missouriensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + maltose
-
Actinoplanes missouriensis ADP + maltose 1-phosphate
-
?

Subunits

Subunits Comment Organism
monomer 1 * 57000, SDS-PAGE Actinoplanes missouriensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
Actinoplanes missouriensis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
additional information
-
the activity of maltokinase increases 2.5fold with a maximum at 55°C, but decreases rapidly at temperatures above 60°C Actinoplanes missouriensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.75
-
in Tris/HCl buffer Actinoplanes missouriensis
8.5
-
in TEA/HCl buffer Actinoplanes missouriensis

Cofactor

Cofactor Comment Organism Structure
ATP dependent on, ATP is not replaceable as phosphoryl-group donor Actinoplanes missouriensis
additional information CTP and TTP show no activity, while GTP with 2.1% activity compared to ATP and UTP with 2.7% show only poor activity as phosphoryl-group donors Actinoplanes missouriensis

pI Value

Organism Comment pI Value Maximum pI Value
Actinoplanes missouriensis isoelectric focusing
-
4.3