2.7.1.160: 2'-phosphotransferase

This is an abbreviated version!
For detailed information about 2'-phosphotransferase, go to the full flat file.

Reaction

Synonyms

2'-phosphotransferase, ApeTpt1, AtPt, CTHT_0025470, HeLa cell 2'-phosphotransferase, human TRPT1, KptA, kptA1, mTPT1, NAD+-dependent 2'-phosphotransferase, NAD-dependent RNA 2'-phosphotransferase, RNA 2'-phosphotransferase, RslTpt1, Tpt1, Tpt1p, tRNA 2'-phosphotransferase, Trpt1, yeast 2'-phosphotransferase

ECTree

     2 Transferases

         2.7 Transferring phosphorus-containing groups

             2.7.1 Phosphotransferases with an alcohol group as acceptor

                2.7.1.160 2'-phosphotransferase

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Results	in table
3237	AA Sequence
1	Application
1	CAS Registry Number
40	Cloned(Commentary)
31	Cofactor
1	Crystallization (Commentary)
112	General Information
34	Inhibitors
17	KM Value [mM]
6	Localization
3	Metals/Ions
4	Molecular Weight [Da]
73	Natural Substrates/ Products (Substrates)
158	Organism
1	Pathway
14	pH Optimum
52	Protein Variants
25	Purification (Commentary)
1	Reaction
3	Reaction Type
135	Reference
13	Source Tissue
3	Specific Activity [micromol/min/mg]
6	Storage Stability
141	Substrates and Products (Substrate)
2	Subunits
240	Synonyms
1	Systematic Name
17	Temperature Optimum [°C]
4	Temperature Range [°C]
17	Turnover Number [1/s]

Temperature Range

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Temperature Range on EC 2.7.1.160 - 2'-phosphotransferase

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TEMPERATURE RANGE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

18 - 30

Saccharomyces cerevisiae

-

F27A, R71Q, TPT1(1-220) and TPT1(1-210) cells failed to grow at 37°C on yeast medium/peptone/dextrose agar, R71Q and TPT1(1-210) strains grew slower than wild-type TPT1

670874

18 - 37

Saccharomyces cerevisiae

-

growth of mutants K16A, K69A, H90A, S91A, H117A, T119A, H142A, R158A and R138K on yeast medium/peptone/dextrose agar, colony sizes were similar to that of wild-type TPT1 cells

670874

25 - 30

Saccharomyces cerevisiae

-

growth of mutants K16A, Y38A, K69A, F72A, H90A, S91A, H117A, T119A, H142A, R158A, TPT1(1-220) and TPT1(1-210) on 5-fluoroorotic acid agar medium

670874

37 - 95

Aeropyrum pernix

Q9YFP5

ApeTpt1 caps the pDNA substrate with high yield (94-100%) at temperatures ranging from 37°C to 65°C and with progressively lower yields at 75°C (68%), 85°C (32%), and 95°C (21%)

762048