2.7.1.160: 2'-phosphotransferase

This is an abbreviated version!
For detailed information about 2'-phosphotransferase, go to the full flat file.

Word Map on EC 2.7.1.160

2.7.1.160

2'-phosphate

junction

adp-ribose

adp-ribosylation

2.7.1.23

phosphodiester

2'-phosphorylated

pre-trnas

Reaction

Synonyms

2'-phosphotransferase, ApeTpt1, AtPt, CTHT_0025470, HeLa cell 2'-phosphotransferase, human TRPT1, KptA, kptA1, mTPT1, NAD+-dependent 2'-phosphotransferase, NAD-dependent RNA 2'-phosphotransferase, RNA 2'-phosphotransferase, RslTpt1, Tpt1, Tpt1p, tRNA 2'-phosphotransferase, Trpt1, yeast 2'-phosphotransferase

ECTree

2 Transferases

2.7 Transferring phosphorus-containing groups

2.7.1 Phosphotransferases with an alcohol group as acceptor

2.7.1.160 2'-phosphotransferase

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Results	in table
3237	AA Sequence
1	Application
1	CAS Registry Number
40	Cloned(Commentary)
31	Cofactor
1	Crystallization (Commentary)
112	General Information
34	Inhibitors
17	KM Value [mM]
6	Localization
3	Metals/Ions
4	Molecular Weight [Da]
73	Natural Substrates/ Products (Substrates)
158	Organism
1	Pathway
14	pH Optimum
52	Protein Variants
25	Purification (Commentary)
1	Reaction
3	Reaction Type
135	Reference
13	Source Tissue
3	Specific Activity [micromol/min/mg]
6	Storage Stability
141	Substrates and Products (Substrate)
2	Subunits
240	Synonyms
1	Systematic Name
17	Temperature Optimum [°C]
4	Temperature Range [°C]
17	Turnover Number [1/s]

Purification

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Purification on EC 2.7.1.160 - 2'-phosphotransferase

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by Blue Sepharose column chromatography

2 entries

by nickel affinity chromatography

Saccharomyces cerevisiae

669189

His6-tagged wild-type RslTpt1 and mutant R16A/H17A/R64A/R119A RslTpt1 from Escherichia coli strain L21(DE3)-CodonPlus by nickel affinity chromatography, ultrafiltration, and gel filtration

Runella slithyformis

762371

immobilized metal ion affinity chromatography, > 80% purified by SDS-PAGE

Saccharomyces cerevisiae

670875

KptA-His6 10% purified by sonication, immobilized metal ion chromatography, centrifugation

Escherichia coli

667569

Ni-agarose chromatography

2 entries

partially purified

3 entries

partially purified as a His6-tagged protein

Saccharomyces cerevisiae

670431

partially purified by Blue sepharose column chromatography

Escherichia coli

670876

partially purified by chromatography on heparin-agarose

Homo sapiens

669180

partially purified, GST-Tpt1 fusion protein purified by glutahthione agarose chromatography

Saccharomyces cerevisiae

670876

purified 28000fold by Orange A Sepharose column, 4fold less pure by streamlined purification procedure

Saccharomyces cerevisiae

Q12272

662096

recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography

4 entries

recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis

2 entries

recombinant wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography

Runella slithyformis

762048

sonication, centrifugation

Aeropyrum pernix

669853

Tpt1-His6 50% purified by sonication, immobilized metal ion chromatography, centrifugation

Saccharomyces cerevisiae

667569