2.7.1.113: deoxyguanosine kinase

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For detailed information about deoxyguanosine kinase, go to the full flat file.

Reaction

Synonyms

(dihydroxypropoxymethyl)guanine kinase, 2'-deoxyguanosine kinase, deoxyadenosine kinase/deoxyguanosine kinase, deoxyguanosine kinase, DG kinase, DGK, dGKase, DGUOK, kinase, deoxyguanosine (phosphorylating), NTP-deoxyguanosine 5'-phosphotransferase, nucleoside triphosphate: deoxyguanosine 5'-phosphotransferase

ECTree

     2 Transferases

         2.7 Transferring phosphorus-containing groups

             2.7.1 Phosphotransferases with an alcohol group as acceptor

                2.7.1.113 deoxyguanosine kinase

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Results	in table
15	Activating Compound
771	AA Sequence
2	Application
1	CAS Registry Number
14	Cloned(Commentary)
33	Cofactor
2	Crystallization (Commentary)
334	Disease/ Diagnostics
2	Expression
9	General Information
9	General Stability
95	Inhibitors
5	kcat/KM [mM/s]
36	Ki Value [mM]
99	KM Value [mM]
32	Localization
47	Metals/Ions
13	Molecular Weight [Da]
39	Natural Substrates/ Products (Substrates)
59	Organism
5	Pathway
18	PDB ID
15	pH Optimum
5	pH Range
3	pH Stability
2	pI Value
42	Protein Variants
9	Purification (Commentary)
5	Reaction
1	Reaction Type
54	Reference
69	Source Tissue
19	Specific Activity [micromol/min/mg]
6	Storage Stability
175	Substrates and Products (Substrate)
8	Subunits
43	Synonyms
1	Systematic Name
6	Temperature Optimum [°C]
1	Temperature Range [°C]
3	Temperature Stability [°C]
23	Turnover Number [1/s]

Purification

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Purification on EC 2.7.1.113 - deoxyguanosine kinase

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PURIFICATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

ammonium sulfate, affinity chromatography on AMP-Sepharose and Blue-Sepharose, DEAE-cellulose, Sephadex G-75

Homo sapiens

-

640553

enzyme activity is stabilized during purification in presence of Triton X-100 and ATP

Homo sapiens

Q16854

661453

mitochondrial deoxyguanosine kinase, one-step purification via deoxyguanosine-3'-(4-aminophenylphosphate)-Sepharose affinity chromatography

Bos taurus

-

640551

mitochondrial deoxyguanosine kinase, Sephacryl S-200, Blue Sepharose CL6B

Bos taurus

-

640550

pH 5.5, ammonium sulfate, DEAE-Sephadex

Sus scrofa

-

640552

pH 5.7, streptomycin sulfate, ammonium sulfate

Mus musculus

-

640549

recombinant deoxyguanosine kinase, BlueA dye affinity, phenyl-Sepharose

Bacillus subtilis

-

640561

recombinant enzyme from Escherichia coli by affinity and phenyl resin chromatography to homogeneity

Bacillus subtilis

-

640561

recombinant enzyme, metal affinity chromatography

Homo sapiens

-

640555