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2.5.1.91: all-trans-decaprenyl-diphosphate synthase

This is an abbreviated version!
For detailed information about all-trans-decaprenyl-diphosphate synthase, go to the full flat file.

Word Map on EC 2.5.1.91

Reaction

(2E,6E)-farnesyl diphosphate
+ 7 isopentenyl diphosphate = 7 diphosphate +
all-trans-decaprenyl diphosphate

Synonyms

AgDPPS, D-less polyprenyl diphosphate synthase, Ddps, ddsa, DdsA protein, decaprenyl diphosphate synthase, decaprenyl diphosphate synthase 1, decaprenyl diphosphate synthase 2, decaprenyl diphosphate synthase gene, decaprenyl-PP synthase, DecPP synthase, decraprenyl diphosphate synthase, DLP1, Dps, DPS1, Rsdds

ECTree

     2 Transferases
         2.5 Transferring alkyl or aryl groups, other than methyl groups
             2.5.1 Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
                2.5.1.91 all-trans-decaprenyl-diphosphate synthase

Cloned

Cloned on EC 2.5.1.91 - all-trans-decaprenyl-diphosphate synthase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
coexpression of decaprenyl diphosphate synthase and 1-deoxy-D-xylulose 5-phosphate synthase in Escherichia coli
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DdsA is expressed successfully in Escherichia coli. Recombinant Escherichia coli synthesizes UQ-10 in addition to the endogenous production UQ-8
efficient production of coenzyme Q10 in rice by introducing the gene for decaprenyl diphosphate synthase into rice sugary and shrunken mutants
expressed under the control of an Escherichia coli constitutive promoter, CoQ10 rather than endogenous CoQ8 is biologically synthesized as the major coenzyme Q. Expression of the ddsA gene with low copy number leads to the accumulation of CoQ10 in batch fermentation. A high cell density in fed-batch fermentation of Escherichia coli BL21/pACDdsA increases the CoQ10 concentration
expression in Escherichia coli
expression in Escherichia coli, detecting UQ-10 in addition to innate UQ-8 in Escherichia coli
expression in Escherichia coli. Escherichia coli JM109 harboring the dps gene produces ubiquinone-10 in addition to endogenous ubiquinone-8
expression in Escherichia coli. Expression of the ddsA gene complements the lethality resulting from a defect in the octaprenyl diphosphate synthase gene of Escherichia coli and produces coenzyme Q10, indicating that coenzyme Q10 can substitute for the function of coenzyme Q8
expression in Escherichia coli. Wild-type Escherichia coli produces coenzyme Q8 and does not produce any coenzyme Q10. Recombinant Escherichia coli produces coenzyme Q10 in addition to coenzyme Q8
for biotechnological production of coenzyme Q10 in recombinant Escherichia coli, three genetic manipulations are performed: heterologous expression of decaprenyl diphosphate synthase (Dps) from Agrobacterium tumefaciens, deletion of endogenous octaprenyl diphosphate synthase (IspB), and overexpression of 1-deoxy-D-xylulose synthase (Dxs). Expression of the dps gene and deletion of the ispB gene in Escherichia coli BL21(DE3) DELTAispB/pAP1 allows production of CoQ10 only. Coexpression of the dxs gene increases the specific content of CoQ10
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gene AgDPPS1, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis, recombinant expression of N-terminally GST-tagged enzyme in Escherichia coli strain BL21, coexpression with gene AgDPPS2
gene AgDPPS2, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis, recombinant expression of N-terminally GST-tagged enzyme in Escherichia coli strain BL21, coexpression with gene AgDPPS1
genetic engineering of rice to produce CoQ10 using the gene for decaprenyl diphosphate synthase from Gluconobacter suboxydans. The production of CoQ9 is almost completely replaced with that of CoQ10, despite the presence of endogenous CoQ9 synthesis. DdsA designed to express at the mitochondria increases accumulation of total CoQ amount in seeds
the proteins Dps1 abd Dlp1 must be present simultaneously in Escherichia coli transformants before ubiquinone-10, which is produced by Schizosaccharomyces pombe but not by Escherichia coli, is generated. DLP1 and DPS1 form a heterotetramer in Escherichia coli