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Literature summary for 2.5.1.91 extracted from

  • Kaur, D.; Brennan, P.J.; Crick, D.C.
    Decaprenyl diphosphate synthesis in Mycobacterium tuberculosis (2004), J. Bacteriol., 186, 7564-7570.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol
-
Mycobacterium tuberculosis
additional information stimulation by detergent Mycobacterium tuberculosis
Triton X-100 maximum activity at 0.1% Mycobacterium tuberculosis

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.029
-
neryl diphosphate pH 7.9, 37°C Mycobacterium tuberculosis
0.04
-
(2E,6E,10E)-geranylgeranyl diphosphate pH 7.9, 37°C Mycobacterium tuberculosis
0.084
-
(2E,6E)-farnesyl diphosphate pH 7.9, 37°C Mycobacterium tuberculosis
0.089
-
isopentenyl diphosphate pH 7.9, 37°C Mycobacterium tuberculosis
0.29
-
(2E,6Z)-farnesyl diphosphate pH 7.9, 37°C Mycobacterium tuberculosis
0.49
-
geranyl diphosphate pH 7.9, 37°C Mycobacterium tuberculosis

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ much less effective than Mg2+ Mycobacterium tuberculosis
Mg2+ maximum activity at 1 mM Mycobacterium tuberculosis
Mn2+ maximum activity at 0.1 mM, about 35% of the activity with Mg2+ Mycobacterium tuberculosis
additional information absolute requirement for divalent kations Mycobacterium tuberculosis
Zn2+ much less effective than Mg2+ Mycobacterium tuberculosis

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,6E)-farnesyl diphosphate + 7 isopentenyl diphosphate
-
Mycobacterium tuberculosis 7 diphosphate + decaprenyl diphosphate
-
?
(2E,6E,10E)-geranylgeranyl diphosphate + isopentenyl diphosphate
-
Mycobacterium tuberculosis diphosphate + octaprenyl diphosphate plus, in decreasing order, nonaprenyl diphosphate, decaprenyl diphosphate, and heptaprenyl diphosphate ?
(2E,6Z)-farnesyl diphosphate + isopentenyl diphosphate highest catalytic efficiency Mycobacterium tuberculosis diphosphate + decaprenyl diphosphate plus smaller amounts of intermediiates with shorter chain length ?
geranyl diphosphate + isopentenyl diphosphate
-
Mycobacterium tuberculosis diphosphate + decaprenyl diphosphate plus some farnesyl diphosphate ?
neryl diphosphate + isopentenyl diphosphate
-
Mycobacterium tuberculosis diphosphate + nonaprenyl diphosphate plus some farnesyl diphosphate ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.03
-
(2E,6E,10E)-geranylgeranyl diphosphate pH 7.9, 37°C Mycobacterium tuberculosis
0.052
-
neryl diphosphate pH 7.9, 37°C Mycobacterium tuberculosis
0.06
-
geranyl diphosphate pH 7.9, 37°C Mycobacterium tuberculosis
0.072
-
(2E,6E)-farnesyl diphosphate pH 7.9, 37°C Mycobacterium tuberculosis
0.08
-
isopentenyl diphosphate pH 7.9, 37°C Mycobacterium tuberculosis
11.5
-
(2E,6Z)-farnesyl diphosphate pH 7.9, 37°C Mycobacterium tuberculosis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8.5
-
Mycobacterium tuberculosis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.122
-
geranyl diphosphate pH 7.9, 37°C Mycobacterium tuberculosis
0.75
-
(2E,6E,10E)-geranylgeranyl diphosphate pH 7.9, 37°C Mycobacterium tuberculosis
0.86
-
(2E,6E)-farnesyl diphosphate pH 7.9, 37°C Mycobacterium tuberculosis
0.89
-
isopentenyl diphosphate pH 7.9, 37°C Mycobacterium tuberculosis
1.79
-
neryl diphosphate pH 7.9, 37°C Mycobacterium tuberculosis
39.65
-
(2E,6Z)-farnesyl diphosphate pH 7.9, 37°C Mycobacterium tuberculosis