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2.4.1.82: galactinol-sucrose galactosyltransferase

This is an abbreviated version!
For detailed information about galactinol-sucrose galactosyltransferase, go to the full flat file.

Word Map on EC 2.4.1.82

Reaction

alpha-D-galactosyl-(1->3)-1D-myo-inositol
+
sucrose
=
myo-inositol
 +
raffinose

Synonyms

alkaline alpha galactosidase 1, At4g01970, galactinol:sucrose 6-galactosyl transferase, galactosyltransferase, galactinol-sucrose, More, raffinose synthase, raffinose synthase 1, raffinose synthase 2, raffinose synthase 3, raffinose synthase 4, raffinose synthase 5, raffinose synthase 6, RafS, RFO synthase/galactosylhydrolase, RFS4, RFS5, RS1, RS2, RS3, RS4, RS5, RS6, ZmRAFS

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.82 galactinol-sucrose galactosyltransferase

Cloned

Cloned on EC 2.4.1.82 - galactinol-sucrose galactosyltransferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Nicotiana tabacum cultivar Yunyan 85
expression in Escherichia coli
functional expression as His-tagged protein in Spodoptera frugiperda Sf21 cells via baculovirus infection
gene RFS, cloned from genomic DNA from seddling leaves, DNA and amino acid sequence determination and analysis, semi-quantitative PCR enzyme expression analysis
gene RFS4, cloning from seeds and functional recombinant expression of His-tagged enzyme in Escherichia coli
gene RFS5, functional recombinant expression in Escherichia coli strain BL21 codon plus
zmrafs, recombinant overexpression of GFP-tagged enzyme in maize protoplasts with or without transcription factor ZmDREB1A, chromatin immunoprecipitation-quantitative PCR enzyme expression analysis and real-time RT-PCR analysis