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Literature summary for 2.4.1.82 extracted from

  • Egert, A.; Keller, F.; Peters, S.
    Abiotic stress-induced accumulation of raffinose in Arabidopsis leaves is mediated by a single raffinose synthase (RS5, At5g40390) (2013), BMC Plant Biol., 13, 218.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.82

Cloned(Commentary)

Cloned (Comment) Organism
gene RFS5, functional recombinant expression in Escherichia coli strain BL21 codon plus Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
additional information construction of two independent loss-of-function mutants for gene RFS5, i.e. rs 5-1 and rs 5-2. Leaves of mutant plants fail to accumulate any raffinose under diverse abiotic stresses including water-deficit, high salinity, heat shock, and methyl viologen-induced oxidative stress. Correlated to the lack of raffinose under these abiotic stress conditions, both mutant plants lack the typical stress-induced raffinose synthase activity increase observed in the leaves of wild-type plants. The seeds of both mutant plants still contain raffinose Phenotypes, overview Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
alpha-D-galactosyl-(1->3)-1D-myo-inositol + sucrose Arabidopsis thaliana
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 myo-inositol + raffinose
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q9FND9 gene RFS5, encoding stress-induced isoform RS5
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf isozyme RS5 Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-D-galactosyl-(1->3)-1D-myo-inositol + sucrose
-
 Arabidopsis thaliana myo-inositol + raffinose
-
 ?

Synonyms

Synonyms Comment Organism
raffinose synthase
-
 Arabidopsis thaliana
RS5
-
 Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Arabidopsis thaliana

Expression

Organism Comment Expression
Arabidopsis thaliana the enzyme is induced in leaves by abiotic stresses up

General Information

General Information Comment Organism
malfunction leaves of RFS5 mutant plants fail to accumulate any raffinose under diverse abiotic stresses including water-deficit, high salinity, heat shock, and methyl viologen-induced oxidative stress. Correlated to the lack of raffinose under these abiotic stress conditions, both mutant plants lack the typical stress-induced raffinose synthase activity increase observed in the leaves of wild-type plants Arabidopsis thaliana
metabolism the enzyme catalyzes the second step in raffinose biosynthesis Arabidopsis thaliana
physiological function the enzyme is responsible for low temperature-induced raffinose accumulation in Arabidopsis thaliana leaves Arabidopsis thaliana