2.4.1.289: N-acetylglucosaminyl-diphospho-decaprenol L-rhamnosyltransferase
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For detailed information about N-acetylglucosaminyl-diphospho-decaprenol L-rhamnosyltransferase, go to the full flat file.
Reaction
Synonyms
cell wall rhamnosyltransferase, Cj1125c, dTDP-Rha:alpha-D-GlcNAc-pyrophosphate polyprenol, alpha-3-L-rhamnosyltransferase, lmo1080, PglA, RmlT, WbbL
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Engineering
Engineering on EC 2.4.1.289 - N-acetylglucosaminyl-diphospho-decaprenol L-rhamnosyltransferase
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additional information
the Escherichia coli mutant strain K12 EMG2 contains an insertionally inactivated wbbL and is devoid of any O-antigen and shows no WbbL activity, complementation by expression of WbbL from Mycobacterium tuberculosis, a plasmid encoding the putative rhamnosyltransferase gene from Mycobacterium tuberculosis also results in O-antigen synthesis
additional information
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the Escherichia coli mutant strain K12 EMG2 contains an insertionally inactivated wbbL and is devoid of any O-antigen and shows no WbbL activity, complementation by expression of WbbL from Mycobacterium tuberculosis, a plasmid encoding the putative rhamnosyltransferase gene from Mycobacterium tuberculosis also results in O-antigen synthesis
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additional information
the temperature-sensitive mutant contains a single-base change that has a proline changed to a serine residue. Exposure of in mc2155 mutant 2-20/32 to higher non-permissive temperatures results in bacteria that cannot be recovered at the lower permissive temperatures. Complementation of the mutants strain by expression of gene wbbL from Escherichia coli or Mycobacterium tuberculosis
additional information
Mycolicibacterium smegmatis mc(2)155 / ATCC 700084
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the temperature-sensitive mutant contains a single-base change that has a proline changed to a serine residue. Exposure of in mc2155 mutant 2-20/32 to higher non-permissive temperatures results in bacteria that cannot be recovered at the lower permissive temperatures. Complementation of the mutants strain by expression of gene wbbL from Escherichia coli or Mycobacterium tuberculosis
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