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2.4.1.289: N-acetylglucosaminyl-diphospho-decaprenol L-rhamnosyltransferase

This is an abbreviated version!
For detailed information about N-acetylglucosaminyl-diphospho-decaprenol L-rhamnosyltransferase, go to the full flat file.

Reaction

dTDP-6-deoxy-beta-L-mannose
+
N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol
=
dTDP
+
alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol

Synonyms

cell wall rhamnosyltransferase, Cj1125c, dTDP-Rha:alpha-D-GlcNAc-pyrophosphate polyprenol, alpha-3-L-rhamnosyltransferase, lmo1080, PglA, RmlT, WbbL

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.289 N-acetylglucosaminyl-diphospho-decaprenol L-rhamnosyltransferase

Cloned

Cloned on EC 2.4.1.289 - N-acetylglucosaminyl-diphospho-decaprenol L-rhamnosyltransferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene pglH, overexpression of His-tagged PglA in Escherichia coli
-
gene wbb, overexpression in Escherichia coli strain BL21(DE3)
-
gene wbbL, expression in and complementation of Mycobacterium smegmatis strain mc2155 temperature-sensitive mutant 2-20/32
orf Rv3265c or gene wbbL, expression in and complementation of Mycobacterium smegmatis strain mc2155 temperature-sensitive mutant 2-20/32, the enzyme also is capable of restoring an Escherichia coli K12 strain containing an insertionally inactivated wbbL to O-antigen positive, functional expression from plasmid in Escherichia coli strain K12 EMG2