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2.4.1.277: 10-deoxymethynolide desosaminyltransferase

This is an abbreviated version!
For detailed information about 10-deoxymethynolide desosaminyltransferase, go to the full flat file.

Word Map on EC 2.4.1.277

Reaction

dTDP-3-dimethylamino-3,4,6-trideoxy-alpha-D-glucopyranose
+
10-deoxymethynolide
=
dTDP
 +
10-deoxymethymycin

Synonyms

DesVII, DesVII/DesVIII, glycosyltransferase DesVII, macrolide glycosyltransferase DesVII

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.277 10-deoxymethynolide desosaminyltransferase

Cloned

Cloned on EC 2.4.1.277 - 10-deoxymethynolide desosaminyltransferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression of desVII asaC-terminally His6-tagged protein in Escherichia coli. When affinity-tagged DesVII and DesVIII proteins are coproduced in Escherichia coli, they form a tight (alphabeta)3 complex that is at least 1000-fold more active than DesVII alone. The formation of the DesVII/DesVIII complex requires coexpression of both genes in vivo and cannot be fully achieved by mixing the individual protein components in vitro
the recombinant DesVII protein is overproduced in Escherichia coli as a C-terminal His6-tagged fusion protein. The DesVIII protein is produced in Escherichia coli as a fusion with maltose-binding protein at its N-terminus. The fusion protein is cleaved by treatment with protease and untagged DesVIII protein is used in the assays together with DesVII