2.4.1.277: 10-deoxymethynolide desosaminyltransferase
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For detailed information about 10-deoxymethynolide desosaminyltransferase, go to the full flat file.
Word Map on EC 2.4.1.277
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2.4.1.277
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venezuelae
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pikromycin
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polyketide
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deoxysugars
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aglycone
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macrolactone
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d-desosamine
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methymycin
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erythromycin
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tylosin
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synthases
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auxiliary
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tylactone
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interspecies
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saccharopolyspora
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medicine
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neomethymycin
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unnatural
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l-rhamnose
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bioconversion
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fradiae
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cassettes
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erythraea
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erythromycin-susceptible
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faecium
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enterococcus
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narbomycin
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aureus
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16-membered
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glycosyltransferases
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synthesis
- 2.4.1.277
- venezuelae
- pikromycin
- polyketide
-
deoxysugars
-
aglycone
-
macrolactone
-
d-desosamine
- methymycin
- erythromycin
- tylosin
- synthases
-
auxiliary
- tylactone
-
interspecies
-
saccharopolyspora
- medicine
- neomethymycin
-
unnatural
- l-rhamnose
-
bioconversion
- fradiae
- cassettes
- erythraea
-
erythromycin-susceptible
- faecium
-
enterococcus
- narbomycin
- aureus
-
16-membered
- glycosyltransferases
- synthesis
Reaction
Synonyms
DesVII, DesVII/DesVIII, glycosyltransferase DesVII, macrolide glycosyltransferase DesVII
ECTree
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Application
Application on EC 2.4.1.277 - 10-deoxymethynolide desosaminyltransferase
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medicine
synthesis
the catalytic flexibility of the glycosyltransferase DesVII offers an means for creating new macrolide antibiotics. The substrate flexibility of DesVII/DesVIII is apparently extended to a wide range of linear as well as cyclic substrates. Although the yield of products is notably reduced when unnatural substrates are used, once a desired activity is identified, the catalytic efficiency of these enzymes may be fine-tuned by protein engineering. The substrate flexibility of glycosyltransferases expands the opportunities for glycodiversification to generate new glycoforms of synthetic compounds and macrolide analogues
medicine
the requirement for an additional protein component, DesVIII, for activity must be taken into consideration in the design of combinatorial biosynthetic experiments with new glycosylated macrolides
expression of different deoxysugar biosynthetic gene cassettes and the gene encoding a substrate-flexible glycosyltransferase DesVII in Streptomyces venezuelae YJ003 mutant strain bearing a deletion of a desosamine biosynthetic (des) gene cluster. The resulting recombinants produce macrolide antibiotic YC-17 analogs possessing unnatural sugars replacing native D-desosamine. These metabolites are D-quinovosyl-10-deoxymethynolide, L-rhamnosyl-10-deoxymethynolide, L-olivosyl-10-deoxymethynolide, and D-boivinosyl-10-deoxymethynolide
synthesis
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expression of different deoxysugar biosynthetic gene cassettes and the gene encoding a substrate-flexible glycosyltransferase DesVII in Streptomyces venezuelae YJ003 mutant strain bearing a deletion of a desosamine biosynthetic (des) gene cluster. The resulting recombinants produce macrolide antibiotic YC-17 analogs possessing unnatural sugars replacing native D-desosamine. These metabolites are D-quinovosyl-10-deoxymethynolide, L-rhamnosyl-10-deoxymethynolide, L-olivosyl-10-deoxymethynolide, and D-boivinosyl-10-deoxymethynolide
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