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2.4.1.222: O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase

This is an abbreviated version!
For detailed information about O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase, go to the full flat file.

Word Map on EC 2.4.1.222

Reaction

UDP-beta-D-GlcNAc
+
PCQNGGS(O-beta-L-fucosyl)-CKDQL
=
UDP
 +
PCQNGGS(O-D-GlcNAcbeta(1-3)-L-Fucbeta)-CKDQL

Synonyms

acetylglucosaminylferase, uridine diphosphoacetylglucosamine:fucosyglycoprotein beta1-3-, beta-1,3-N-acetyl-glucosaminyltransferase, beta-1,3-N-acetylglucosaminyltransferase, beta1,3-acetylglucosaminyltransferase, beta1,3-N-acetylglucosaminyltransferase, beta1,3-N-acetylglucosaminyltransferase-2, beta1,3-N-acetylglusoaminyltransferase, Dfng, FNG, Fringe, fringe beta1,3 N-acetylglucosaminyltransferase, fringe glycosyltransferase, Fringe O-fucose-beta1,3-N-acetylglucosaminyltransferase, Fringe protein, glycosyltransferase Fringe, LFNG, Lunatic Fringe, lunatic fringe glycosyltransferase, Maniac Fringe, Manic fringe, manic fringe glycosyltransferase, MFng, N-acetylglucosaminyltransferase, O-fucose-beta1,3-N-acetylglucosaminyltransferase, O-fucosylpeptide beta-1,3-N-acetylglucosaminyltransferase, O-fucosylpeptide-3-beta-N-acetylglucosaminyltransferase, Radical Fringe, radical fringe glycosyltransferase, Rfng, UDP-acetylglucosamine:fucosylglycoprotein, UDP-D-GlcNAc:O-L-fucosylpeptide 3-beta-N-acetyl-D-glucosaminyltransferase, UDP-GlcNAc:fucose beta1,3 N-acetylglucosaminyltransferase, UDP-glucose:O-linked fucose beta1,3-glucosyltransferase

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.222 O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase

Engineering

Engineering on EC 2.4.1.222 - O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D327N
EMS mutagenesis, transheterozygote, mutation on allele 14, lethal phenotype
E220K
EMS mutagenesis, transheterozygote, mutation on allele 3, lethal phenotype
G295D
EMS mutagenesis, transheterozygote, mutation on allele L81, lethal phenotype
K196E
EMS mutagenesis, transheterozygote, mutation on allele 11, lethal phenotype
K196N
EMS mutagenesis, transheterozygote, mutation on allele 11, lethal phenotype
L213F
EMS mutagenesis, transheterozygote, mutation on allele L83, lethal phenotype
L398Q
EMS mutagenesis, transheterozygote, mutation on allele 2, phenotype overview
S350T
EMS mutagenesis, transheterozygote, mutation on allele 1, lethal phenotype
T184M
EMS mutagenesis, transheterozygote, phenotype overview
A175V
short loop mutant
A235Y
long loop mutant
C290S
active site mutant to short loop side of residue D289
D288A
active site mutant to short loop side of residue D289, activity too low to measure
D289E
active site mutant, catalytically inactive
E237A
long loop mutant
F251S
active site mutant, catalytically inactive
F251Y
active site mutant to short loop side of residue D289
G254A
active site mutant to short loop side of residue D289
G334H
donor specificity mutant, 94.6% of wild-type UDP-beta-D-GlcNAc utilization, 78.0% of wild-type UDP-Glc utilization
H171A
short loop mutant
H171D
short loop mutant
H313A
donor specificity mutant, 6.2% of wild-type UDP-beta-D-GlcNAc utilization, 75.9% of wild-type UDP-Glc utilization
H313A/G334H
donor specificity mutant, 3.1% of wild-type UDP-beta-D-GlcNAc utilization, 34.7% of wild-type UDP-Glc utilization
I233A
long loop mutant
L176A
short loop mutant
L229Q
long loop mutant
L314R
donor specificity mutant, 21.7% of wild-type UDP-beta-D-GlcNAc utilization, 119.5% of wild-type UDP-Glc utilization
S128V
short loop mutant
S168A
short loop mutant
S228A
active site mutant to short loop side of residue D289
S228L
active site mutant, catalytically inactive
s228T
active site mutant to short loop side of residue D289
S228Y
active site mutant, catalytically inactive
S312T
donor specificity mutant, 1.9% of wild-type UDP-beta-D-GlcNAc utilization, 18.1% of wild-type UDP-Glc utilization
T253A
active site mutant, catalytically inactive
additional information