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Literature summary for 2.4.1.222 extracted from

  • Luther, K.B.; Schindelin, H.; Haltiwanger, R.S.
    Structural and mechanistic insights into lunatic fringe from a kinetic analysis of enzyme mutants (2009), J. Biol. Chem., 284, 3294-3305.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.222

Protein Variants

Protein Variants Comment Organism
A175V short loop mutant Mus musculus
A235Y long loop mutant Mus musculus
C290S active site mutant to short loop side of residue D289 Mus musculus
D288A active site mutant to short loop side of residue D289, activity too low to measure Mus musculus
D289E active site mutant, catalytically inactive Mus musculus
E237A long loop mutant Mus musculus
F251S active site mutant, catalytically inactive Mus musculus
F251Y active site mutant to short loop side of residue D289 Mus musculus
G254A active site mutant to short loop side of residue D289 Mus musculus
G334H donor specificity mutant, 94.6% of wild-type UDP-beta-D-GlcNAc utilization, 78.0% of wild-type UDP-Glc utilization Mus musculus
H171A short loop mutant Mus musculus
H171D short loop mutant Mus musculus
H313A donor specificity mutant, 6.2% of wild-type UDP-beta-D-GlcNAc utilization, 75.9% of wild-type UDP-Glc utilization Mus musculus
H313A/G334H donor specificity mutant, 3.1% of wild-type UDP-beta-D-GlcNAc utilization, 34.7% of wild-type UDP-Glc utilization Mus musculus
I233A long loop mutant Mus musculus
L176A short loop mutant Mus musculus
L229Q long loop mutant Mus musculus
L314R donor specificity mutant, 21.7% of wild-type UDP-beta-D-GlcNAc utilization, 119.5% of wild-type UDP-Glc utilization Mus musculus
S128V short loop mutant Mus musculus
S168A short loop mutant Mus musculus
S228A active site mutant to short loop side of residue D289 Mus musculus
S228L active site mutant, catalytically inactive Mus musculus
s228T active site mutant to short loop side of residue D289 Mus musculus
S228Y active site mutant, catalytically inactive Mus musculus
S312T donor specificity mutant, 1.9% of wild-type UDP-beta-D-GlcNAc utilization, 18.1% of wild-type UDP-Glc utilization Mus musculus
T253A active site mutant, catalytically inactive Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
UDP
-
 Mus musculus
UMP
-
 Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00116
-
 p-nitrophenyl-alpha-L-fucose mutant S168V, pH 6.8, 37°C Mus musculus
0.0109
-
 p-nitrophenyl-alpha-L-fucose wild-type, pH 6.8, 37°C Mus musculus
0.0177
-
 p-nitrophenyl-alpha-L-fucose mutant G254A, pH 6.8, 37°C Mus musculus
0.0182
-
 p-nitrophenyl-alpha-L-fucose mutant H171D, pH 6.8, 37°C Mus musculus
0.0204
-
 p-nitrophenyl-alpha-L-fucose mutant S168A, pH 6.8, 37°C Mus musculus
0.0277
-
 p-nitrophenyl-alpha-L-fucose mutant A175V, pH 6.8, 37°C Mus musculus
0.0343
-
 UDP-beta-D-GlcNAc mutant S168A, pH 6.8, 37°C Mus musculus
0.03633
-
 p-nitrophenyl-alpha-L-fucose mutant E237A, pH 6.8, 37°C Mus musculus
0.0379
-
 UDP-beta-D-GlcNAc wild-type, pH 6.8, 37°C Mus musculus
0.0497
-
 UDP-beta-D-GlcNAc mutant S168V, pH 6.8, 37°C Mus musculus
0.0507
-
 p-nitrophenyl-alpha-L-fucose mutant H171A, pH 6.8, 37°C Mus musculus
0.0577
-
 p-nitrophenyl-alpha-L-fucose mutant I233A, pH 6.8, 37°C Mus musculus
0.0595
-
 p-nitrophenyl-alpha-L-fucose mutant S228T, pH 6.8, 37°C Mus musculus
0.0609
-
 UDP-beta-D-GlcNAc mutant H171D, pH 6.8, 37°C Mus musculus
0.0614
-
 p-nitrophenyl-alpha-L-fucose mutant A235Y, pH 6.8, 37°C Mus musculus
0.0707
-
 UDP-beta-D-GlcNAc mutant A175V, pH 6.8, 37°C Mus musculus
0.111
-
 p-nitrophenyl-alpha-L-fucose mutant C290S, pH 6.8, 37°C Mus musculus
0.113
-
 p-nitrophenyl-alpha-L-fucose mutant L229Q, pH 6.8, 37°C Mus musculus
0.1177
-
 p-nitrophenyl-alpha-L-fucose mutant S228A, pH 6.8, 37°C Mus musculus
0.129
-
 p-nitrophenyl-alpha-L-fucose mutant F251Y, pH 6.8, 37°C Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus O09008 isoform lunatic fringe
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
p-nitrophenyl-alpha-L-fucose + UDP-beta-D-GlcNAc
-
 Mus musculus GlcNAc-beta-1,3-fucitol + p-nitrophenol + UDP
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.08
-
 UDP-beta-D-GlcNAc wild-type, pH 6.8, 37°C Mus musculus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.011
-
 UDP wild-type, pH 6.8, 37°C Mus musculus
0.096
-
 UMP wild-type, pH 6.8, 37°C Mus musculus