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2.3.1.168: dihydrolipoyllysine-residue (2-methylpropanoyl)transferase

This is an abbreviated version!
For detailed information about dihydrolipoyllysine-residue (2-methylpropanoyl)transferase, go to the full flat file.

Word Map on EC 2.3.1.168

Reaction

2-methylpropanoyl-CoA
+
enzyme N6-(dihydrolipoyl)lysine
=
CoA
 +
enzyme N6-(S-[2-methylpropanoyl]dihydrolipoyl)lysine

Synonyms

Bkd, BKDH, DBT, dihydrolipoamide branched chain transacylase, dihydrolipoyl transacetylase, DLD, E2, E2p, More

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.1 Transferring groups other than aminoacyl groups
                2.3.1.168 dihydrolipoyllysine-residue (2-methylpropanoyl)transferase

Cloned

Cloned on EC 2.3.1.168 - dihydrolipoyllysine-residue (2-methylpropanoyl)transferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloning of full length E2 and fragments, expression in Escherichia coli JM109 as fusion protein
-
DNA and amino acid sequence determination anad analysis
DNA sequence determination, overexpression of wild-type, lipol-free apo-enzyme, and mutants in Escherichia coli
-
expressed as recombinant protein in Escherichia coli
-
expression in Escherichia coli
-
expression of apo-enzyme in Escherichia coli strain XL1-Blue, can be lipoylated in vitro
-
expression of E2c, residues 161-421, fused to maltose-binding protein, which enhances the yield of the recombinant enzyme, in Escherichia coli
-
gene contains a mitochondrial targeting presequence
gene E2, DNA sequence determination and analysis
gene E2, mapping to chromosome 1p31
pseudogene E2, DNA sequence determination and analysis, retroposon, gene corresponds to the complete mitochondrial presequence and the lipoyl-bearing domain encoded by exon I through IV of the functional gene E2