2.3.1.140: rosmarinate synthase
This is an abbreviated version!
For detailed information about rosmarinate synthase, go to the full flat file.
Word Map on EC 2.3.1.140
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2.3.1.140
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ammonia-lyase
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officinalis
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lamiaceae
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salvia
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melissa
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coleus
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caffeic
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blumei
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phenylpropanoids
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balm
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4-coumarate
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dracocephalum
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shikimic
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4-hydroxyphenyllactate
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lemon
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solenostemon
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lithospermic
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kotschyi
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synthesis
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miltiorrhiza
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boiss
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coumaric
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smras
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verticillata
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phenylpropanoid-derived
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benth
- 2.3.1.140
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ammonia-lyase
- officinalis
- lamiaceae
- salvia
- melissa
-
coleus
-
caffeic
- blumei
-
phenylpropanoids
- balm
- 4-coumarate
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dracocephalum
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shikimic
- 4-hydroxyphenyllactate
- lemon
-
solenostemon
-
lithospermic
- kotschyi
- synthesis
- miltiorrhiza
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boiss
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coumaric
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smras
- verticillata
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phenylpropanoid-derived
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benth
Reaction
Synonyms
4-coumaroyl-CoA:4-hydroxyphenyllactic acid 4-coumaroyl transferase, 4-coumaroyl-CoA:hydroxyphenyllactic acid hydroxycinnamoyltransferase, AAT1, AT1, caffeoyl-coenzyme A:3,4-dihydroxyphenyllactic acid caffeoyltransferase, hydroxycinnamoyl-CoA:hydroxyphenyllactate hydroxycinnamoyltransferase, RAS, rosmarinic acid synthase
ECTree
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Application
Application on EC 2.3.1.140 - rosmarinate synthase
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synthesis
Coleus scutellarioides
expression of rosmarinic acid synthase from Coleus blumei, 4-coumarate: CoA ligase from Arabidopsis thaliana, 4-hydroxyphenyllactate 3-hydroxylase from Escherichia coli and D-lactate dehydrogenase from Lactobacillus pentosus in an L-tyrosine over-producing Escherichia coli strain leads to a yield of rosmarinic acid of 130 mg/l. In addition, intermediate caffeoyl-phenyllactate is produced at about 55 mg/l
synthesis
Coleus scutellarioides
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production of rosmarinic acid analogues in Escherichia coli by expression of 4-coumarate: CoA ligase from Arabidopsis thaliana and rosmarinic acid synthase from Coleus blumei. Incubation of the recombinant strain with exogenously supplied phenyllactic acid and analogues, and coumaric acid and analogues as donor substrates leads to production of 18 compounds, including unnatural analogues