2.3.1.140: rosmarinate synthase
This is an abbreviated version!
For detailed information about rosmarinate synthase, go to the full flat file.
Word Map on EC 2.3.1.140
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2.3.1.140
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ammonia-lyase
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officinalis
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lamiaceae
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salvia
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melissa
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coleus
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caffeic
-
blumei
-
phenylpropanoids
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balm
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4-coumarate
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dracocephalum
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shikimic
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4-hydroxyphenyllactate
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lemon
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solenostemon
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lithospermic
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kotschyi
-
synthesis
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miltiorrhiza
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boiss
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coumaric
-
smras
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verticillata
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phenylpropanoid-derived
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benth
- 2.3.1.140
-
ammonia-lyase
- officinalis
- lamiaceae
- salvia
- melissa
-
coleus
-
caffeic
- blumei
-
phenylpropanoids
- balm
- 4-coumarate
-
dracocephalum
-
shikimic
- 4-hydroxyphenyllactate
- lemon
-
solenostemon
-
lithospermic
- kotschyi
- synthesis
- miltiorrhiza
-
boiss
-
coumaric
-
smras
- verticillata
-
phenylpropanoid-derived
-
benth
Reaction
Synonyms
4-coumaroyl-CoA:4-hydroxyphenyllactic acid 4-coumaroyl transferase, 4-coumaroyl-CoA:hydroxyphenyllactic acid hydroxycinnamoyltransferase, AAT1, AT1, caffeoyl-coenzyme A:3,4-dihydroxyphenyllactic acid caffeoyltransferase, hydroxycinnamoyl-CoA:hydroxyphenyllactate hydroxycinnamoyltransferase, RAS, rosmarinic acid synthase
ECTree
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KM Value
KM Value on EC 2.3.1.140 - rosmarinate synthase
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0.035
Coleus scutellarioides
-
pH 7.0, 30°C, with 4-coumaroyl-CoA, recombinant RAS
0.0853
3-(3,4-Dihydroxyphenyl)lactate
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with 4-coumaroyl-CoA, pH 7.0, 30°C, crude recombinant His-tagged enzyme
0.1528
3-(3,4-Dihydroxyphenyl)lactate
-
with 4-coumaroyl-CoA, pH 7.0, 30°C, native crude enzyme
0.193
3-(3,4-Dihydroxyphenyl)lactate
Coleus scutellarioides
-
pH 7.0, 30°C, with caffeoyl-CoA, recombinant RAS
0.2126
3-(3,4-Dihydroxyphenyl)lactate
-
with caffeoyl-CoA, pH 7.0, 30°C, crude recombinant His-tagged enzyme
0.2336
3-(3,4-Dihydroxyphenyl)lactate
-
with caffeoyl-CoA, pH 7.0, 30°C, native crude enzyme
0.34
3-(3,4-Dihydroxyphenyl)lactate
Coleus scutellarioides
-
pH 7.0, 30°C, with cinnamoyl-CoA, recombinant RAS
1.3
3-(3,4-Dihydroxyphenyl)lactate
Coleus scutellarioides
-
pH 7.0, 30°C, with feroyl-CoA, recombinant RAS
0.0169
-
with 4-coumaroyl-CoA, pH 7.0, 30°C, native crude enzyme
0.0189
3-(4-hydroxyphenyl)lactate
-
with 4-coumaroyl-CoA, pH 7.0, 30°C, crude recombinant His-tagged enzyme
0.021
3-(4-hydroxyphenyl)lactate
with 4-coumaroyl-CoA, pH 7.5, 30°C, recombinant AAT1
0.025
3-(4-hydroxyphenyl)lactate
Coleus scutellarioides
-
pH 7.0, 30°C, with 4-coumaroyl-CoA, recombinant RAS
0.049
3-(4-hydroxyphenyl)lactate
with caffeoyl-CoA, pH 7.5, 30°C, recombinant AAT1
0.074
3-(4-hydroxyphenyl)lactate
-
with caffeoyl-CoA, pH 7.0, 30°C, native crude enzyme
0.1565
3-(4-hydroxyphenyl)lactate
-
with caffeoyl-CoA, pH 7.0, 30°C, crude recombinant His-tagged enzyme
0.163
3-(4-hydroxyphenyl)lactate
Coleus scutellarioides
-
pH 7.0, 30°C, with caffeoyl-CoA, recombinant RAS
0.55
3-(4-hydroxyphenyl)lactate
Coleus scutellarioides
-
pH 7.0, 30°C, with cinnamoyl-CoA, recombinant RAS
0.652
3-(4-hydroxyphenyl)lactate
with cinnamoyl-CoA, pH 7.5, 30°C, recombinant AAT1
0.672
3-(4-hydroxyphenyl)lactate
with feruloyl-CoA, pH 7.5, 30°C, recombinant AAT1
1.425
3-(4-hydroxyphenyl)lactate
Coleus scutellarioides
-
pH 7.0, 30°C, with feroyl-CoA, recombinant RAS
0.0013
Coleus scutellarioides
-
pH 7.0, 30°C, with 3-(3,4-dihydroxyphenyl)lactate, native RAS
0.0018
4-coumaroyl-CoA
Coleus scutellarioides
-
pH 7.0, 30°C, with 3-(3,4-dihydroxyphenyl)lactate, recombinant RAS
0.0032
4-coumaroyl-CoA
-
with 3-(3,4-dihydroxyphenyl)lactate, pH 7.0, 30°C, crude recombinant His-tagged enzyme
0.0047
4-coumaroyl-CoA
Coleus scutellarioides
-
pH 7.0, 30°C, with 3-(4-hydroxyphenyl)lactate, native RAS
0.0048
4-coumaroyl-CoA
-
with 3-(4-hydroxyphenyl)lactate, pH 7.0, 30°C, native crude enzyme
0.0073
4-coumaroyl-CoA
-
with 3-(3,4-dihydroxyphenyl)lactate, pH 7.0, 30°C, native crude enzyme
0.0092
4-coumaroyl-CoA
Coleus scutellarioides
-
pH 7.0, 30°C, with 3-(4-hydroxyphenyl)lactate, recombinant RAS
0.0094
4-coumaroyl-CoA
-
with 3-(4-hydroxyphenyl)lactate, pH 7.0, 30°C, crude recombinant His-tagged enzyme
0.0107
4-coumaroyl-CoA
Coleus scutellarioides
-
pH 7.0, 30°C, with D-phenyllactate, recombinant RAS
0.014
4-coumaroyl-CoA
with 3-(4-hydroxyphenyl)lactate, pH 7.5, 30°C, recombinant AAT1
0.0817
4-coumaroyl-CoA
Coleus scutellarioides
-
pH 7.0, 30°C, with D-tyrosine, recombinant RAS
0.083
4-coumaroyl-CoA
with shikimate, pH 7.5, 30°C, recombinant AAT1
0.0021
Coleus scutellarioides
-
pH 7.0, 30°C, with 3-(4-hydroxyphenyl)lactate, recombinant RAS
0.0023
caffeoyl-CoA
-
with 3-(4-hydroxyphenyl)lactate, pH 7.0, 30°C, crude recombinant His-tagged enzyme
0.0033
caffeoyl-CoA
-
with 3-(3,4-dihydroxyphenyl)lactate, pH 7.0, 30°C, crude recombinant His-tagged enzyme
0.0034
caffeoyl-CoA
Coleus scutellarioides
-
pH 7.0, 30°C, with 3-(4-hydroxyphenyl)lactate, native RAS
0.0035
caffeoyl-CoA
Coleus scutellarioides
-
pH 7.0, 30°C, with D-phenyllactate, recombinant RAS
0.0039
caffeoyl-CoA
Coleus scutellarioides
-
pH 7.0, 30°C, with 3-(3,4-dihydroxyphenyl)lactate, recombinant RAS
0.004
caffeoyl-CoA
Coleus scutellarioides
-
pH 7.0, 30°C, with 3-(3,4-dihydroxyphenyl)lactate, native RAS
0.0052
caffeoyl-CoA
-
with 3-(3,4-dihydroxyphenyl)lactate, pH 7.0, 30°C, native crude enzyme
0.0058
caffeoyl-CoA
-
with 3-(4-hydroxyphenyl)lactate, pH 7.0, 30°C, native crude enzyme
0.05
caffeoyl-CoA
with 3-(4-hydroxyphenyl)lactate, pH 7.5, 30°C, recombinant AAT1
0.0158
Coleus scutellarioides
-
pH 7.0, 30°C, with 3-(3,4-dihydroxyphenyl)lactate, recombinant RAS
0.025
cinnamoyl-CoA
with 3-(4-hydroxyphenyl)lactate, pH 7.5, 30°C, recombinant AAT1
0.0255
cinnamoyl-CoA
Coleus scutellarioides
-
pH 7.0, 30°C, with 3-(4-hydroxyphenyl)lactate, recombinant RAS
0.0529
cinnamoyl-CoA
Coleus scutellarioides
-
pH 7.0, 30°C, with D-phenyllactate, recombinant RAS
0.49
Coleus scutellarioides
-
pH 7.0, 30°C, with 4-coumaroyl-CoA, recombinant RAS
1.346
D-phenyllactate
Coleus scutellarioides
-
pH 7.0, 30°C, with cinnamoyl-CoA, recombinant RAS
17.82
D-phenyllactate
Coleus scutellarioides
-
pH 7.0, 30°C, with caffeoyl-CoA, recombinant RAS
0.0191
Coleus scutellarioides
-
pH 7.0, 30°C, with 3-(3,4-dihydroxyphenyl)lactate, recombinant RAS
0.022
feruloyl-CoA
with 3-(4-hydroxyphenyl)lactate, pH 7.5, 30°C, recombinant AAT1
0.0364
feruloyl-CoA
Coleus scutellarioides
-
pH 7.0, 30°C, with 3-(4-hydroxyphenyl)lactate, recombinant RAS