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2.3.1.133: shikimate O-hydroxycinnamoyltransferase

This is an abbreviated version!
For detailed information about shikimate O-hydroxycinnamoyltransferase, go to the full flat file.

Word Map on EC 2.3.1.133

Reaction

4-coumaroyl-CoA
+
shikimate
=
CoA
 +
4-coumaroylshikimate

Synonyms

CST, HCS/QT, HCT, HCT1, HCT1806, HCT2, HCT2a, HCT4918, HQT, HQT1, HQT2, HST, hydroxycinnamoyl CoA quinate/shikimate hydroxycinnamoyl transferase, hydroxycinnamoyl CoA:shikimate hydroxycinnamoyl transferase, hydroxycinnamoyl-CoA shikimate/quinate hydroxycinnamoyltransferase, hydroxycinnamoyl-CoA:quinate hydroxycinnamoyltransferase, hydroxycinnamoyl-CoA:shikimate hydroxycinnamoyl transferase, hydroxycinnamoyl-CoA:shikimate hydroxycinnamoyl-transferase, hydroxycinnamoyl-CoA:shikimate hydroxycinnamoyltransferase, hydroxycinnamoyl-CoA:shikimate O-hydroxycinnamoyl transferase, hydroxycinnamoyl-CoA:shikimate/quinate hydroxycinnamoyltransferase, hydroxycinnamoyl-coenzyme A shikimate/quinate hydroxycinnamoyltransferase, hydroxycinnamoyltransferase, hydroxycinnamoyltransferase, shikimate, OsHCT4, p-coumaroyl shikimate transferase, p-coumaroyl-CoA:5-O-shikimate p-coumaroyl transferase, p-coumaroyl-CoA:shikimic acid p-coumaroyl transferase, p-hydroxycinnamoyl-CoA:shikimate-p-hydroxycinnamoyl transferase, shikimate hydroxycinnamoyltransferase

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.1 Transferring groups other than aminoacyl groups
                2.3.1.133 shikimate O-hydroxycinnamoyltransferase

Cloned

Cloned on EC 2.3.1.133 - shikimate O-hydroxycinnamoyltransferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
an antisense construct is generated for down-regulation of HCT
-
expressed in Escherichia coli
expression in Escherichia coli
expression of GST-tagged HCT in Escherichia coli strain BL21 (DE3)
-
gene cbhct2, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of His-tagged HST in Escherichia coli strain BL21(DE3)pLysS
Coleus scutellarioides
gene OsHCT4, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression as soluble GST-tagged enzyme using vector pGEX 5X-1 in Escherichia coli strain BL21(DE3)
-
genes PeHCT-1, PeHCT-2, PeHCT-3, PeHCT-4, PeHCT-5, PeHCT-6, and PeHCT-7, expression as GST-tagged enzymes in Escherichia coli strain BL21 (DE3)
-
overexpression of N-terminally His6-tagged HCT in Escherichia coli strain BL21 Star(DE3)pLysS using a commercially available synthetic gene encoding HCT with codons optimized for Escherichia coli expression
recombinantly expressed in Escherichia coli
the amplified PCR fragments of the putative HCT cDNA clone are sequenced and cloned into a derivative of pAHC25, the resulting plasmid containing the HCT RNAi construct is named pHF5
-