2.3.1.133: shikimate O-hydroxycinnamoyltransferase

This is an abbreviated version!
For detailed information about shikimate O-hydroxycinnamoyltransferase, go to the full flat file.

Reaction

Synonyms

CST, HCS/QT, HCT, HCT1, HCT1806, HCT2, HCT2a, HCT4918, HQT, HQT1, HQT2, HST, hydroxycinnamoyl CoA quinate/shikimate hydroxycinnamoyl transferase, hydroxycinnamoyl CoA:shikimate hydroxycinnamoyl transferase, hydroxycinnamoyl-CoA shikimate/quinate hydroxycinnamoyltransferase, hydroxycinnamoyl-CoA:quinate hydroxycinnamoyltransferase, hydroxycinnamoyl-CoA:shikimate hydroxycinnamoyl transferase, hydroxycinnamoyl-CoA:shikimate hydroxycinnamoyl-transferase, hydroxycinnamoyl-CoA:shikimate hydroxycinnamoyltransferase, hydroxycinnamoyl-CoA:shikimate O-hydroxycinnamoyl transferase, hydroxycinnamoyl-CoA:shikimate/quinate hydroxycinnamoyltransferase, hydroxycinnamoyl-coenzyme A shikimate/quinate hydroxycinnamoyltransferase, hydroxycinnamoyltransferase, hydroxycinnamoyltransferase, shikimate, OsHCT4, p-coumaroyl shikimate transferase, p-coumaroyl-CoA:5-O-shikimate p-coumaroyl transferase, p-coumaroyl-CoA:shikimic acid p-coumaroyl transferase, p-hydroxycinnamoyl-CoA:shikimate-p-hydroxycinnamoyl transferase, shikimate hydroxycinnamoyltransferase

ECTree

     2 Transferases

         2.3 Acyltransferases

             2.3.1 Transferring groups other than aminoacyl groups

                2.3.1.133 shikimate O-hydroxycinnamoyltransferase

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Results	in table
4	Activating Compound
243	AA Sequence
4	Application
1	CAS Registry Number
15	Cloned(Commentary)
3	Crystallization (Commentary)
1	Expression
12	General Information
1	General Stability
18	Inhibitors
93	KM Value [mM]
3	Localization
4	Metals/Ions
15	Molecular Weight [Da]
19	Natural Substrates/ Products (Substrates)
35	Organism
12	Pathway
13	PDB ID
19	pH Optimum
8	pH Range
1	pH Stability
1	pI Value
6	Protein Variants
15	Purification (Commentary)
2	Reaction
3	Reaction Type
34	Reference
35	Source Tissue
17	Specific Activity [micromol/min/mg]
7	Storage Stability
136	Substrates and Products (Substrate)
7	Subunits
51	Synonyms
1	Systematic Name
13	Temperature Optimum [°C]
1	Temperature Range [°C]
1	Temperature Stability [°C]

Crystallization

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Crystallization on EC 2.3.1.133 - shikimate O-hydroxycinnamoyltransferase

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CRYSTALLIZATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

purified recombinant HCT, sitting drop technique, mixing of 100 nl of 20 mg/ml protein in 20 mM Tris-HCl, pH 7.5, 150 mM NaCl, 5 mM 2-mercaptoethanol, with 100 nl of precipitant solution containing 1.6 M magnesium sulfate, 0.1 M MES pH 6.5, 20°C, 2 months, X-ray diffraction structure determination and analysis at 3.0 A resolution, molecular replacement

Coffea canephora

A4ZKE4

718519

in complex with CoA and 4-coumaroyl-shikimate. The 4-coumaroyl-shikimate contacts the enzyme via the phenolic group and carbonyl group of the 4-coumaroyl portion. The shikimate portion contacts the enzyme through both the carboxyl and hydroxyl groups. In the protocatechuate ternary complex, protocatechuate binds in a very similar manner to shikimate, with the carboxyl group making a tight salt bridge with Arg369, and the C3 hydroxyl group interacting with the nitrogen NE2 of His163

Panicum virgatum

R9RYW2

736977

structures in its apo-form and ternary complex with shikimate and 4-coumaroyl-CoA, which is converted to its product during crystal soaking. Residues threonine36, serine38, tyrosine40, histidine162, arginine371, and threonine384 are involved in catalysis and specificity. Histidine162 and threonine36 play a role in the catalytic mechanism. Substrate binding should occur sequentially, with 4-coumaroyl-CoA binding prior to the acyl acceptor molecule. Comparison of the structure of sorghum HCT with the HCT involved in chlorogenic acid synthesis in Coffea canephora

Sorghum bicolor

C5XXB7

737024