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2.3.1.133: shikimate O-hydroxycinnamoyltransferase

This is an abbreviated version!
For detailed information about shikimate O-hydroxycinnamoyltransferase, go to the full flat file.

Word Map on EC 2.3.1.133

Reaction

4-coumaroyl-CoA
+
shikimate
=
CoA
+
4-coumaroylshikimate

Synonyms

CST, HCS/QT, HCT, HCT1, HCT1806, HCT2, HCT2a, HCT4918, HQT, HQT1, HQT2, HST, hydroxycinnamoyl CoA quinate/shikimate hydroxycinnamoyl transferase, hydroxycinnamoyl CoA:shikimate hydroxycinnamoyl transferase, hydroxycinnamoyl-CoA shikimate/quinate hydroxycinnamoyltransferase, hydroxycinnamoyl-CoA:quinate hydroxycinnamoyltransferase, hydroxycinnamoyl-CoA:shikimate hydroxycinnamoyl transferase, hydroxycinnamoyl-CoA:shikimate hydroxycinnamoyl-transferase, hydroxycinnamoyl-CoA:shikimate hydroxycinnamoyltransferase, hydroxycinnamoyl-CoA:shikimate O-hydroxycinnamoyl transferase, hydroxycinnamoyl-CoA:shikimate/quinate hydroxycinnamoyltransferase, hydroxycinnamoyl-coenzyme A shikimate/quinate hydroxycinnamoyltransferase, hydroxycinnamoyltransferase, hydroxycinnamoyltransferase, shikimate, OsHCT4, p-coumaroyl shikimate transferase, p-coumaroyl-CoA:5-O-shikimate p-coumaroyl transferase, p-coumaroyl-CoA:shikimic acid p-coumaroyl transferase, p-hydroxycinnamoyl-CoA:shikimate-p-hydroxycinnamoyl transferase, shikimate hydroxycinnamoyltransferase

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.1 Transferring groups other than aminoacyl groups
                2.3.1.133 shikimate O-hydroxycinnamoyltransferase

Purification

Purification on EC 2.3.1.133 - shikimate O-hydroxycinnamoyltransferase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
crude extracts from stem material from various down-regulated and control lines are assayed for extractable HCT enzyme activity
-
partial
recombinant enzyme
recombinant GST-tagged HCT from Escherichia coli strain BL21 (DE3) by glutathione affinity chromatography
-
recombinant GST-tagged PeHCT-1, PeHCT-2, PeHCT-3, PeHCT-4, PeHCT-5, PeHCT-6, and PeHCT-7 gene products from Escherichia coli strain BL21 (DE3) by glutathione affinity cromatography
-
recombinant His-tagged HST from Escherichia coli strain BL21(DE3)pLysS by nickel affinity chromatography and dialysis
Coleus scutellarioides
recombinant N-terminally His6-tagged HCT from Escherichia coli strain BL21 Star(DE3)pLysS by nickel affinity chromatography, dialysis, and cleavage of the tag by TEV protease, followed by gel filtration
soluble recombinant GST-tagged OsHCT4 from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography
-
tracheary elements are cultured
-