2.1.1.264: 23S rRNA (guanine2069-N7)-methyltransferase
This is an abbreviated version!
For detailed information about 23S rRNA (guanine2069-N7)-methyltransferase, go to the full flat file.
Reaction
Synonyms
3S rRNA m7G2069 methyltransferase, bifunctional methyltransferase, m2G2069 methyltransferase, ribosomal large subunit methyltransferase, rlmK, RlmKL, SAV1081, Smu472, ycbY
ECTree
Advanced search results
Engineering
Engineering on EC 2.1.1.264 - 23S rRNA (guanine2069-N7)-methyltransferase
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
D195A
-
site-directed mutagenesis, the mutation does not affect the methylation activity
D568A
-
site-directed mutagenesis, the D568A mutation in the C-terminal domain does not rescue m7G2069 formation, but m2G2445 is efficiently formed in this mutant
D597A
-
site-directed mutagenesis, the mutation does not affect the methylation activity
N309A
-
site-directed mutagenesis, the N309A mutation in the NTD impairs m2G2445 formation, but rescues m7G2069 formation
N397A
-
site-directed mutaagenesis, the N397A mutant exhibits no m2G2445 formation but rescues m7G2069 formation
R530A
-
site-directed mutagenesis, the mutation does not affect the methylation activity
additional information
-
generation of a ycbY knock-out strain, completely inactive in methylation, from Escherichia coli strain BW25113 and complementation with active ycbY
additional information
-
generation of a ycbY knock-out strain, completely inactive in methylation, from Escherichia coli strain BW25113 and complementation with active ycbY
-