Information on EC 2.1.1.264 - 23S rRNA (guanine2069-N7)-methyltransferase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.1.1.264
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RECOMMENDED NAME
GeneOntology No.
23S rRNA (guanine2069-N7)-methyltransferase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
S-adenosyl-L-methionine + guanine2069 in 23S rRNA = S-adenosyl-L-homocysteine + N7-methylguanine2069 in 23S rRNA
show the reaction diagram
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SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:23S rRNA (guanine2069-N7)-methyltransferase
The enzyme specifically methylates guanine2069 at position N7 in 23S rRNA. In gamma-proteobacteria the enzyme also catalyses EC 2.1.1.173, 23S rRNA (guanine2445-N2)-methyltransferase, while in beta-proteobacteria the activities are carried out by separate proteins [1]. The enzyme from the gamma-proteobacterium Escherichia coli has RNA unwinding activity as well [1].
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
gene rlmKL or ycbY
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Manually annotated by BRENDA team
gene rlmK
UniProt
Manually annotated by BRENDA team
gene rlmK
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
malfunction
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + guanine2069 in 23S rRNA
S-adenosyl-L-homocysteine + N7-methylguanine2069 in 23S rRNA
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + guanine2069 in 23S rRNA
S-adenosyl-L-homocysteine + N7-methylguanine2069 in 23S rRNA
show the reaction diagram
additional information
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
S-adenosyl-L-methionine
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5
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assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
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assay at
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
purified recombinant YcbY, in presence of S-adenosyl-L-methionine and S-adenosyl-L-homocysteine, hanging drop or sitting drop vapor diffusion method, 16°C, X-ray diffraction structure determination and analysis
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crystal structure analysis, overview
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purified recombinant Smu472, in presence of S-adenosyl-L-methionine and S-adenosyl-L-homocysteine, hanging drop or sitting drop vapor diffusion method, 16°C, X-ray diffraction structure determination and analysis
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant His-tagged Smu472 from Escherichiac coli strain BL21(DE3) by nickel affinity chromatography
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recombinant His-tagged YcbY from Escherichiac coli strain BL21(DE3) by nickel affinity chromatography
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression of His-tagged Smu472 in Escherichiac coli strain BL21(DE3)
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gene rlmK, expression in Escherichia coli DELTArlmKL mutant
gene rlmL, renamed rlmKL, encodes a fused methyltransferase responsible for forming both m7G2069 and m2G2445
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gene ycbY, DNA and amino acid sequence determination and analysis, expression of His-tagged YcbY in Escherichiac coli strain BL21(DE3)
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D195A
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site-directed mutagenesis, the mutation does not affect the methylation activity
D568A
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site-directed mutagenesis, the D568A mutation in the C-terminal domain does not rescue m7G2069 formation, but m2G2445 is efficiently formed in this mutant
D597A
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site-directed mutagenesis, the mutation does not affect the methylation activity
N309A
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site-directed mutagenesis, the N309A mutation in the NTD impairs m2G2445 formation, but rescues m7G2069 formation
N397A
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site-directed mutaagenesis, the N397A mutant exhibits no m2G2445 formation but rescues m7G2069 formation
R530A
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site-directed mutagenesis, the mutation does not affect the methylation activity
additional information
Show AA Sequence (2784 entries)
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