2.1.1.255: geranyl diphosphate 2-C-methyltransferase
This is an abbreviated version!
For detailed information about geranyl diphosphate 2-C-methyltransferase, go to the full flat file.
Word Map on EC 2.1.1.255
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2.1.1.255
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cyclization
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2-methylisoborneol
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terpenoids
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c-methylation
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taste
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monoterpene
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coelicolor
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carbocation
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odor
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actinomycete
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isoprenoids
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cyanobacteria
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terpene
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sam-dependent
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limnetica
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s-adenosyl-l-methionine-dependent
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unpleasant
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dimethylallyl
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farnesyl
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pseudanabaena
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t-butyl
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ethylbenzene
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intermediacy
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lanosterol
- 2.1.1.255
-
cyclization
- 2-methylisoborneol
-
terpenoids
-
c-methylation
-
taste
-
monoterpene
- coelicolor
-
carbocation
-
odor
-
actinomycete
-
isoprenoids
- cyanobacteria
-
terpene
-
sam-dependent
- limnetica
-
s-adenosyl-l-methionine-dependent
-
unpleasant
-
dimethylallyl
-
farnesyl
-
pseudanabaena
-
t-butyl
- ethylbenzene
-
intermediacy
- lanosterol
Reaction
Synonyms
2-methyl-GPP synthase, C-methyl transferase, geranyl diphosphate 2-methyltransferase, geranyl diphosphate C-methyltransferase, geranyl pyrophosphate methyltransferase, GPP 2-methyl transferase, GPP methyltransferase, GPPMT, MGPPS, MTF, SCO7701, SCO7701 protein
ECTree
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Crystallization
Crystallization on EC 2.1.1.255 - geranyl diphosphate 2-C-methyltransferase
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purified recombinant His6-tagged wild-type enzyme in complex with S-adenosyl-L-methionine and monoterpenoid ligands geranyl-S-thiolodiphosphate, a substrate analogue, or geranyl diphosphate, sitting drop vapor diffusion method, 4°C, mixing of 0.001 ml protein solution conatining 6 mg/mL enzyme, 50 mM PIPES, pH 6.7, 20% glycerol, 5 mM BME, 15 mM MgCl2, 100 mM NaCl, 10 mM S-adenosyl-L-methionine, and 1 mM monoterpenoid ligand with 0.001 ml of precipitant solution containing 100 mM HEPES, pH 7.5, 25% polyethylene glycol 3350, and 200 mM (NH4)2SO4 for cocrystallization with geranyl-S-thiolodiphosphate, 100 mM Bis-Tris, pH 6.5, 25% polyethylene glycol 3350, and 200 mM (NH4)2SO4 for cocrystallization with geranyl diphosphate and equilibrated against a 0.1 ml reservoir of precipitant solution at 21°C, 1 day, X-ray diffraction structure determination and analysis at 2.05 A resolution
purified recombinant His6-tagged apo-GPPMT containing one molecule in the asymmetric unit, or in ternary complex with geranyl diphosphate and sinefungin, hanging drop method, mixing of protein solution containing 5 mg/ml in 50 mM sodium phosphate, pH 7.5, 300 mM NaCl and 10% glycerol, with S-adenosyl-L-methionine at a protein:S-adenosyl-L-methionine molar ratio of 1:7, 4°C, and further with reservoir solution containing 50 mM sodium cacodylate, pH 6.5, 1.4 M lithium sulfate, 30 mM MgCl2, and 1 mM spermine for GPPMT-GPP-SFG form I, or 50 mM sodium cacodylate, pH 6.7, 1.3 M lithium sulfate, 30 mM MgCl2, and 1 mM spermine for GPPMT-GPP-SFG form II, method screening, X-ray diffraction structure determination and analysis at 1.7-3.5 A resolution, molecular replacement method