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2.1.1.255: geranyl diphosphate 2-C-methyltransferase

This is an abbreviated version!
For detailed information about geranyl diphosphate 2-C-methyltransferase, go to the full flat file.

Word Map on EC 2.1.1.255

Reaction

S-adenosyl-L-methionine
+
geranyl diphosphate
=
S-adenosyl-L-homocysteine
+
(E)-2-methylgeranyl diphosphate

Synonyms

2-methyl-GPP synthase, C-methyl transferase, geranyl diphosphate 2-methyltransferase, geranyl diphosphate C-methyltransferase, geranyl pyrophosphate methyltransferase, GPP 2-methyl transferase, GPP methyltransferase, GPPMT, MGPPS, MTF, SCO7701, SCO7701 protein

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.255 geranyl diphosphate 2-C-methyltransferase

Purification

Purification on EC 2.1.1.255 - geranyl diphosphate 2-C-methyltransferase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant His6-tagged enzyme from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography and gel filtration
recombinant N-terminally His6-tagged soluble SCO7700 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography to homogeneity and over 95% purity
soluble recombinant His6-tagged wild-type and mutant geranyl diphosphate methyltransferase from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration