Cloned (Comment) | Organism |
---|---|
expression of His6-tagged enzyme in Escherichia coli strain BL21 (DE3) | Streptomyces lasalocidi |
overproduced in Escherichia coli strain BL21 | Streptomyces lasalocidi |
Crystallization (Comment) | Organism |
---|---|
purified recombinant His6-tagged apo-GPPMT containing one molecule in the asymmetric unit, or in ternary complex with geranyl diphosphate and sinefungin, hanging drop method, mixing of protein solution containing 5 mg/ml in 50 mM sodium phosphate, pH 7.5, 300 mM NaCl and 10% glycerol, with S-adenosyl-L-methionine at a protein:S-adenosyl-L-methionine molar ratio of 1:7, 4°C, and further with reservoir solution containing 50 mM sodium cacodylate, pH 6.5, 1.4 M lithium sulfate, 30 mM MgCl2, and 1 mM spermine for GPPMT-GPP-SFG form I, or 50 mM sodium cacodylate, pH 6.7, 1.3 M lithium sulfate, 30 mM MgCl2, and 1 mM spermine for GPPMT-GPP-SFG form II, method screening, X-ray diffraction structure determination and analysis at 1.7-3.5 A resolution, molecular replacement method | Streptomyces lasalocidi |
sitting-drop vapour-diffusion method | Streptomyces lasalocidi |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + geranyl diphosphate | Streptomyces lasalocidi | methylation of geranyl diphosphate before the cyclization reaction | S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces lasalocidi | D3KYU3 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Streptomyces lasalocidi |
recombinant His6-tagged enzyme from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography and gel filtration | Streptomyces lasalocidi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + geranyl diphosphate | methylation of geranyl diphosphate before the cyclization reaction | Streptomyces lasalocidi | S-adenosyl-L-homocysteine + (E)-2-methylgeranyl diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
geranyl pyrophosphate methyltransferase | - |
Streptomyces lasalocidi |
GPP methyltransferase | - |
Streptomyces lasalocidi |
GPPMT | - |
Streptomyces lasalocidi |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Streptomyces lasalocidi |