2.1.1.251: methylated-thiol-coenzyme M methyltransferase

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For detailed information about methylated-thiol-coenzyme M methyltransferase, go to the full flat file.

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Synonyms

480-kDa CoM methylase, CoM methylase, DMS:CoM methyltransferase, methylthiol:coenzyme M methyltransferase, methylthiol:CoM methyltransferase, methyltransferase II, MMPA:CoM methyltransferase, MtsA

ECTree

     2 Transferases

         2.1 Transferring one-carbon groups

             2.1.1 Methyltransferases

                2.1.1.251 methylated-thiol-coenzyme M methyltransferase

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Results	in table
3	Activating Compound
20	AA Sequence
1	CAS Registry Number
2	Cloned(Commentary)
2	Cofactor
13	General Information
1	Inhibitors
5	KM Value [mM]
3	Metals/Ions
5	Molecular Weight [Da]
14	Natural Substrates/ Products (Substrates)
10	Organism
5	Pathway
2	pH Optimum
2	Purification (Commentary)
4	Reaction
6	Reference
1	Renatured (Commentary)
6	Source Tissue
1	Specific Activity [micromol/min/mg]
32	Substrates and Products (Substrate)
1	Subunits
25	Synonyms
1	Systematic Name
4	Temperature Optimum [°C]

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RENATURED/Commentary

ORGANISM

UNIPROT

LITERATURE

recombinant enzyme from inclusion bodies harvested from cells solubilized using 6 M guanidine hydrochloride, 50 mM DTT, and 20 mM 2-mercaptoethanol. The solubilized protein is diluted 60fold with protein refolding at room temperature for 24 h in refolding buffer consisting of 500 mM Tris, pH 8.0, 1 M KCl, 10 mM DTT, 10 mM 2-mercaptoethanol, and 100 mM ZnCl2. Refolding buffers containing 500 mM guanidine hydrochloride, 33 mM CHAPS, 0.5% Triton X-100, 5 mM SDS, or 20% glycerol do not produce active methyltransferase, nor do refolding at 4°C in the optimal refolding buffer

Methanosarcina barkeri

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717763