2.1.1.183: 18S rRNA (adenine1779-N6/adenine1780-N6)-dimethyltransferase

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For detailed information about 18S rRNA (adenine1779-N6/adenine1780-N6)-dimethyltransferase, go to the full flat file.

Word Map on EC 2.1.1.183

2.1.1.183

pombe

schizosaccharomyces

tri-snrnps

mitosis

pre-rrnas

nucleolar

fission

collagen

ribonucleoprotein

oncogene

spindle

thioredoxin-like

pre-ribosomal

anaphase-promoting

metastasis-associated

salivary

pirin

spliceosomal

transketolase

v-ha-ras

adenoid

cyclosome

snrnp

cystic

Reaction

4 S-adenosyl-L-methionine +

= 4 S-adenosyl-L-homocysteine +

N6-dimethyladenine1779/N6-dimethyladenine1780 in 18S rRNA

Synonyms

18S rRNA dimethylase Dim1p, DIM1, Dim1p, EC 2.1.1.48, KIDIM1, m2(6)A dimethylase, ScDim1

ECTree

2 Transferases

2.1 Transferring one-carbon groups

2.1.1 Methyltransferases

2.1.1.183 18S rRNA (adenine1779-N6/adenine1780-N6)-dimethyltransferase

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Results	in table
25	AA Sequence
4	Cloned(Commentary)
5	General Information
3	Localization
2	Molecular Weight [Da]
3	Natural Substrates/ Products (Substrates)
6	Organism
14	PDB ID
2	Protein Variants
1	Purification (Commentary)
1	Reaction
6	Reference
8	Substrates and Products (Substrate)
2	Subunits
10	Synonyms
1	Systematic Name

Engineering

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Engineering on EC 2.1.1.183 - 18S rRNA (adenine1779-N6/adenine1780-N6)-dimethyltransferase

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Go to Protein Variants Search

E85A

Saccharomyces cerevisiae

C7GSG6

the altered protein is able to complement for Dim1 but yields a protein with no catalytic activity as determined by primer extension of 18S rRNA. The expected primer extension stops at 1779 and 1780 are missing in rRNA extracted from the strain expressing catalytically inactive ScDim1. The single mutant shows no change in growth rate of yeast at 18, 25, 30, and 37°C

705155

additional information

Saccharomyces cerevisiae

C7GSG6

to delineate regions of the eukaryotic Dim1 critical to its function, KsgA/Dim1 chimeras are created and tested. Construction of six chimeras that result from swapping the two domains of Saccharomyces cerevisiae Dim1, Escherichia coli KsgA, and Methanocaldococcus jannaschii Dim1. Of the chimeras, only one constructed with the N-terminal domain from eukaryotic Dim1 and the C-terminal domain from archaeal Dim1 is able to complement for the eukaryotic Dim1, suggesting that eukaryotic-specific Dim1 function resides in the N-terminal domain, where few structural differences are observed between members of the KsgA/Dim1 family

705155