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2.1.1.183: 18S rRNA (adenine1779-N6/adenine1780-N6)-dimethyltransferase

This is an abbreviated version!
For detailed information about 18S rRNA (adenine1779-N6/adenine1780-N6)-dimethyltransferase, go to the full flat file.

Word Map on EC 2.1.1.183

Reaction

4 S-adenosyl-L-methionine +

adenine1779/adenine1780 in 18S rRNA
= 4 S-adenosyl-L-homocysteine +
N6-dimethyladenine1779/N6-dimethyladenine1780 in 18S rRNA

Synonyms

18S rRNA dimethylase Dim1p, DIM1, Dim1p, EC 2.1.1.48, KIDIM1, m2(6)A dimethylase, ScDim1

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.183 18S rRNA (adenine1779-N6/adenine1780-N6)-dimethyltransferase

Substrates Products

Substrates Products on EC 2.1.1.183 - 18S rRNA (adenine1779-N6/adenine1780-N6)-dimethyltransferase

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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
4 S-adenosyl-L-methionine + adenine1779/1780 in 18S rRNA
4 S-adenosyl-L-homocysteine + N6-dimethyladenine1779/N6-dimethyladenine1780 in 18S rRNA
show the reaction diagram
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?
4 S-adenosyl-L-methionine + adenine1779/adenine1780 in 18S rRNA
4 S-adenosyl-L-homocysteine + N6-dimethyladenine1779/N6-dimethyladenine1780 in 18S rRNA
show the reaction diagram
4 S-adenosyl-L-methionine + adenine1779/adenine1780 in 18S rRNA
4 S-adenosyl-L-homocysteine + N6-dimethyladenosine1779/N6-dimethyladenine1780 in 18S rRNA
show the reaction diagram
isolation of mutations that uncouple the enzymatic function of Dim1p in methylation from its involvement in prerRNA processing
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?
4 S-adenosyl-L-methionine + adenine1779/adenine1780 in 18S rRNA
4 S-adenosyl-L-homocysteine + N6-dimethyladenosine1779/N6-dimethyladenosine1780 in 18S rRNA
show the reaction diagram
neither the bacterial nor the archaeal ortholog could complement for the eukaryotic Dim1. This might be related to the secondary, non-methyltransferase function that Dim1 is known to play in eukaryotic ribosomal maturation
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?
additional information
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the enzyme catalyzed methylation of Escherichia coli 30S ribosomes. The enzyme produces a mixture of N6-methyladenine and 6-dimethyladenine (1.4 times as much N6-methyladenine as N6-dimethyladenine). 80% of the potential sites are methylated after 2 h. Under the assay conditions, ScDim1 releases the N6-methyladenine intermediate, which is subsequently converted to the N6-dimethyladenine product after an additional binding event
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?