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2.1.1.173: 23S rRNA (guanine2445-N2)-methyltransferase

This is an abbreviated version!
For detailed information about 23S rRNA (guanine2445-N2)-methyltransferase, go to the full flat file.

Word Map on EC 2.1.1.173

Reaction

S-adenosyl-L-methionine
+
guanine2445 in 23S rRNA
=
S-adenosyl-L-homocysteine
 +
N2-methylguanine2445 in 23S rRNA

Synonyms

23S RNA m(2)G2445 methyltransferase, EC 2.1.1.51, EC 2.1.1.52, m2G2445 methyltransferase, ribosomal large subunit methyltransferase, RlmKL, RlmL, Smu472, ycbY

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.173 23S rRNA (guanine2445-N2)-methyltransferase

Crystallization

Crystallization on EC 2.1.1.173 - 23S rRNA (guanine2445-N2)-methyltransferase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
purified recombinant His6-tagged RlmL, hanging drop vapour diffusion method, mixing of 0.002 ml of 20 mg/ml protein in 20 mM Tris-HCl, pH 8.0, S-adenosyl-L-homocysteine-saturated, and 200 mM NaCl, with 0.002 ml of reservoir solution containing 0.1 M HEPES, pH 7.0, and 12% w/v PEG 8000, and with 0.001 ml of 30%(w/v) n-octanoylsucrose solution, addition of 2 mM S-adenosyl-L-methionine, equilibration against 0.5 ml of reservoir solution, method optimization, X-ray diffraction structure determination and analysis at 2.2 A resolution