Literature summary for 2.1.1.173 extracted from

Wang, K.T.; Ma, L.; Nan, J.; Su, X.D.; Li, L.
Purification, crystallization and preliminary X-ray crystallographic analysis of 23S RNA m(2)G2445 methyltransferase RlmL from Escherichia coli (2010), Acta Crystallogr. Sect. F, 66, 1484-1486.

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Cloned(Commentary)

Cloned (Comment)	Organism
gene rlmL, expression of recombinant His6-tagged RlmL in Escherichia coli strain BL21(DE3)	Escherichia coli

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant His6-tagged RlmL, hanging drop vapour diffusion method, mixing of 0.002 ml of 20 mg/ml protein in 20 mM Tris-HCl, pH 8.0, S-adenosyl-L-homocysteine-saturated, and 200 mM NaCl, with 0.002 ml of reservoir solution containing 0.1 M HEPES, pH 7.0, and 12% w/v PEG 8000, and with 0.001 ml of 30%(w/v) n-octanoylsucrose solution, addition of 2 mM S-adenosyl-L-methionine, equilibration against 0.5 ml of reservoir solution, method optimization, X-ray diffraction structure determination and analysis at 2.2 A resolution	Escherichia coli

Crystallization (Comment)

Organism

purified recombinant His6-tagged RlmL, hanging drop vapour diffusion method, mixing of 0.002 ml of 20 mg/ml protein in 20 mM Tris-HCl, pH 8.0, S-adenosyl-L-homocysteine-saturated, and 200 mM NaCl, with 0.002 ml of reservoir solution containing 0.1 M HEPES, pH 7.0, and 12% w/v PEG 8000, and with 0.001 ml of 30%(w/v) n-octanoylsucrose solution, addition of 2 mM S-adenosyl-L-methionine, equilibration against 0.5 ml of reservoir solution, method optimization, X-ray diffraction structure determination and analysis at 2.2 A resolution

Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
78900	-	x * 78900, about, sequence calculation, x * 82000, recombinant His6-tagged enzyme, SDS-PAGE	Escherichia coli
82000	-	x * 78900, about, sequence calculation, x * 82000, recombinant His6-tagged enzyme, SDS-PAGE	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
S-adenosyl-L-methionine + guanine2445 in 23S rRNA	Escherichia coli	-	S-adenosyl-L-homocysteine + N2-methylguanine2445 in 23S rRNA	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P75864	gene rlmL, previously known as ycbY	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His6-tagged RlmL from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration to homogeneity	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
S-adenosyl-L-methionine + guanine2445 in 23S rRNA	-	Escherichia coli	S-adenosyl-L-homocysteine + N2-methylguanine2445 in 23S rRNA	-	?

Subunits

Subunits	Comment	Organism
?	x * 78900, about, sequence calculation, x * 82000, recombinant His6-tagged enzyme, SDS-PAGE	Escherichia coli

Synonyms

Synonyms	Comment	Organism
23S RNA m(2)G2445 methyltransferase	-	Escherichia coli
RlmL	-	Escherichia coli
ycbY	-	Escherichia coli

Cofactor

Cofactor	Comment	Organism	Structure
S-adenosyl-L-methionine	dependent on	Escherichia coli