2.1.1.173: 23S rRNA (guanine2445-N2)-methyltransferase
This is an abbreviated version!
For detailed information about 23S rRNA (guanine2445-N2)-methyltransferase, go to the full flat file.
Word Map on EC 2.1.1.173
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2.1.1.173
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ether
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n-acetylmuramic
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rrnas
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plasmalogens
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6-phosphate
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coa
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murnac
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haemophilus
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renamed
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peptidoglycan
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citrate
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glucokinase
- 2.1.1.173
- ether
-
n-acetylmuramic
- rrnas
-
plasmalogens
-
6-phosphate
- coa
- murnac
-
haemophilus
-
renamed
- peptidoglycan
- citrate
- glucokinase
Reaction
Synonyms
23S RNA m(2)G2445 methyltransferase, EC 2.1.1.51, EC 2.1.1.52, m2G2445 methyltransferase, ribosomal large subunit methyltransferase, RlmKL, RlmL, Smu472, ycbY
ECTree
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Substrates Products
Substrates Products on EC 2.1.1.173 - 23S rRNA (guanine2445-N2)-methyltransferase
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REACTION DIAGRAM
S-adenosyl-L-methionine + guanine2069 in 23S rRNA
S-adenosyl-L-homocysteine + N7-methylguanine2069 in 23S rRNA
S-adenosyl-L-methionine + guanine2445 in 23S rRNA
S-adenosyl-L-homocysteine + N2-methylguanine2445 in 23S rRNA
S-adenosyl-L-homocysteine + N7-methylguanine2069 in 23S rRNA
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S-adenosyl-L-methionine + guanine2069 in 23S rRNA
S-adenosyl-L-homocysteine + N7-methylguanine2069 in 23S rRNA
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S-adenosyl-L-methionine + guanine2069 in 23S rRNA
S-adenosyl-L-homocysteine + N7-methylguanine2069 in 23S rRNA
m7G2069 modification by YcbY is stoichiometric
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S-adenosyl-L-homocysteine + N2-methylguanine2445 in 23S rRNA
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S-adenosyl-L-methionine + guanine2445 in 23S rRNA
S-adenosyl-L-homocysteine + N2-methylguanine2445 in 23S rRNA
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S-adenosyl-L-methionine + guanine2445 in 23S rRNA
S-adenosyl-L-homocysteine + N2-methylguanine2445 in 23S rRNA
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S-adenosyl-L-methionine + guanine2445 in 23S rRNA
S-adenosyl-L-homocysteine + N2-methylguanine2445 in 23S rRNA
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S-adenosyl-L-methionine + guanine2445 in 23S rRNA
S-adenosyl-L-homocysteine + N2-methylguanine2445 in 23S rRNA
N2-methylguanosine2445 of the 23S rRNA is located in a cluster of modified nucleotides concentrated at the peptidyl transferase center of the ribosome. It is likely that the G2445 modification is necessary for prevention of nonfunctional secondary or tertiary structure formation at the peptidyl transferase center
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S-adenosyl-L-methionine + guanine2445 in 23S rRNA
S-adenosyl-L-homocysteine + N2-methylguanine2445 in 23S rRNA
recombinant YcbY protein is able to methylate 23S rRNA purified from the ycbY knock-out strain in vitro, assembled 50S subunits are not a substrate for the methylase
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S-adenosyl-L-methionine + guanine2445 in 23S rRNA
S-adenosyl-L-homocysteine + N2-methylguanine2445 in 23S rRNA
RlmL possessing an additional RNA-binding domain acts on naked ribosomal RNA or early assembly intermediates in the cell
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S-adenosyl-L-methionine + guanine2445 in 23S rRNA
S-adenosyl-L-homocysteine + N2-methylguanine2445 in 23S rRNA
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duplex formation of H74 is not required for the m2G2445 formation. The 29-mer single-stranded transcript 6, which consists of residues C2422 to A2450, can form m2G2445 efficiently
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S-adenosyl-L-methionine + guanine2445 in 23S rRNA
S-adenosyl-L-homocysteine + N2-methylguanine2445 in 23S rRNA
m2G2445 modification by YcbY is stoichiometric
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S-adenosyl-L-methionine + guanine2445 in 23S rRNA
S-adenosyl-L-homocysteine + N2-methylguanine2445 in 23S rRNA
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S-adenosyl-L-methionine + guanine2445 in 23S rRNA
S-adenosyl-L-homocysteine + N2-methylguanine2445 in 23S rRNA
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Smu472 adds the m2G2445 modification
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the bifunctional methyltransferase YcbY, i.e. RlmKL, adds the m7G2069 and m2G2445 modifications in Escherichia coli 23S rRNA, recognition of dual rRNA targets by YcbY
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additional information
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the bifunctional methyltransferase YcbY, i.e. RlmKL, adds the m7G2069 and m2G2445 modifications in Escherichia coli 23S rRNA, recognition of dual rRNA targets by YcbY
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additional information
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helix 80 and the 12 nt ss region are critical sites necessary for m2G2445 and m7G2069 formation. Transcript 7, which lacks helix 80 and the 12 nt single-strand region, is not methylated at either position
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additional information
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Smu776 does not target G2069, and this nucleotide remains unmodified in Gram-positive rRNAs
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