1.3.1.3: DELTA4-3-oxosteroid 5beta-reductase
This is an abbreviated version!
For detailed information about DELTA4-3-oxosteroid 5beta-reductase, go to the full flat file.
Reaction
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Synonyms
3-oxo-DELTA4-steroid 5beta-reductase, 4,5beta-dihydrocortisone:NADP+ DELTA4-oxidoreductase, 5-beta-reductase, 5beta-POR, 5beta-red, AKR1D1, AKR1D4, aldo-keto reductase, aldo-keto reductase 1D1, aldo–keto reductase 1D1, androstenedione 5beta-reductase, CARUB_v10005016mg, cortisone 5beta-reductase, cortisone beta-reductase, DELTA 4-5beta-reductase, DELTA4-3-ketosteroid 5beta-reductase, DELTA4-3-oxosteroid 5beta-reductase, DELTA4-hydrogenase, EC 1.3.1.23, EC 1.3.1.3, h5beta-red, h5beta-reductase, More, P5betaR, progesterone 5beta-reductase, reductase, cholestenone 5beta.-, reductase, cortisone DELTA4-5beta-, SRD5beta, St5betaR1, steroid 5beta-reductase, steroid 5beta.-reductase, testosterone 5-beta-reductase, testosterone 5beta-reductase
ECTree
Purification
Purification on EC 1.3.1.3 - DELTA4-3-oxosteroid 5beta-reductase
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by Ni-NTA affinity chromatography
cortisone 5beta-reduction (EC 1.3.1.3) and androstenedione reduction (EC 1.3.1.23) are catalyzed by the same catalytic site of a single protein
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glutathione-Sepharose column chromatography and Sephacryl-S100 gel filtration
Ni2+-Sepharose column chromatography
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nickel-Sepharose affinity column chromatography and Superdex 75 gel filtration
recombinant AKR1D1 enzyme mutant P133R from HEK-293 cells, the other expressed mutants cannot be purified
recombinant GST-tagged enzyme from Escherichia coli by glutathione affinity and anion exchange chromatography, and gel filtration
recombinant wild-type and mutant enzymes from Escherichia coli strain M15 by nickel affinity chromatography
to apparent homogeneity by affinity chromatography
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to homogeneity by Ni–NTA affinity chromatography
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by Ni-NTA affinity chromatography
by Ni-NTA affinity chromatography