Literature summary for 1.3.1.3 extracted from

Di Costanzo, L.; Drury, J.E.; Penning, T.M.; Christianson, D.W.
Crystal structure of human liver DELTA4-3-ketosteroid 5beta-reductase (AKR1D1) and implications for substrate binding and catalysis (2008), J. Biol. Chem., 283, 16830-16839.

Search for more literature for 1.3.1.3

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli strain C41(DE3)	Homo sapiens

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging drop vapour diffusion method, in 0.1 M Tris/HEPES (pH 7.5), 10-20% (w/v) polyethylene glycol 4000, and 10% isopropyl alcohol	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
E120A	mutant is devoid of activity	Homo sapiens
Y58F	mutant is devoid of activity	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
testosterone	-	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0027	-	testosterone	in 100 mM potassium phosphate buffer (pH 6.0), at 37°C	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni2+-Sepharose column chromatography	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
liver	-	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
cortisone + NADPH + H+	-	Homo sapiens	5beta-17,21dihydroxy-pregnan-3,11,20-trione + NADP+	-	?
progesterone + NADPH + H+	-	Homo sapiens	5beta-pregnane-3,20-dione + NADP+	-	?
testosterone + NADPH + H+	-	Homo sapiens	(5beta,17beta)-17-hydroxyandrostan-3-one + NADP+	-	?

Synonyms

Synonyms	Comment	Organism
AKR1D1	-	Homo sapiens
DELTA4-3-ketosteroid 5beta-reductase	-	Homo sapiens
steroid 5beta-reductase	-	Homo sapiens

Cofactor

Cofactor	Comment	Organism	Structure
NADPH	-	Homo sapiens

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.0145	-	testosterone	in 100 mM potassium phosphate buffer (pH 6.0), at 37°C	Homo sapiens

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Release 2023.1 (January 2023)