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Literature summary for 1.3.1.3 extracted from
- Molecular cloning and heterologous expression of progesterone 5beta-reductase from Digitalis lanata Ehrh. (2006), Phytochemistry, 67, 225-231.
Application
| Application | Comment | Organism |
|---|---|---|
| additional information | 5beta-POR is highly conserved within the genus Digitalis and the respective genes and proteins share considerable homology to putative progesterone reductases from other plant species | Digitalis lanata |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| Sph1/Sal1 5beta-POR fragment cloned into the pQE vector and transformed into Escherichia coli strain M15(pREP4) | Digitalis lanata |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.008 | - |
NADPH | - |
Digitalis lanata |  |
| 0.12 | - |
progesterone | - |
Digitalis lanata | |
| 0.228 | - |
4-androstene-3,17-dione | - |
Digitalis lanata | |
| 0.291 | - |
cortisol | - |
Digitalis lanata | |
| 1.597 | - |
cortexone | - |
Digitalis lanata | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
x * 40000, SDS–PAGE | Digitalis lanata |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Digitalis lanata | Q6PQJ9 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| by Ni-NTA affinity chromatography | Digitalis lanata |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Digitalis lanata | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-androstene-3,17-dione + NADPH | - |
Digitalis lanata | 5beta-androstan-3,17-dione + NADP+ | - |
? | |
| cortexone + NADPH | - |
Digitalis lanata | 21-trihydroxy-5beta-pregnane-3,20-dione + NADP+ | - |
? | |
| cortisol + NADPH | - |
Digitalis lanata | 11beta,17,21-trihydroxy-5beta-pregnane-3,20-dione + NADP+ | - |
? | |
| additional information | pregnenolone, 21-OH-pregenenolone and isoprogesterone are not accepted by recombinant 5beta-POR | Digitalis lanata | ? | - |
? | |
| progesterone + NADPH + H+ | 5alpha-pregnane-3,20-dione, the 5alpha-isomer of the pregnane-3,20-dione is not formed | Digitalis lanata | 5beta-pregnane-3,20-dione + NADP+ | - |
ir | |
| testosterone + NADPH + H+ | - |
Digitalis lanata | (5beta,17beta)-17-hydroxyandrostan-3-one + NADP+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 40000, SDS–PAGE | Digitalis lanata |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 5beta-POR | - |
Digitalis lanata |
| progesterone 5beta-reductase | - |
Digitalis lanata |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
with progesterone as substrate | Digitalis lanata |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | - |
with progesterone as substrate | Digitalis lanata |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | cannot be replaced by NADH | Digitalis lanata | |
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