1.17.7.3: (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase (flavodoxin)
This is an abbreviated version!
For detailed information about (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase (flavodoxin), go to the full flat file.
Word Map on EC 1.17.7.3
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1.17.7.3
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e-4-hydroxy-3-methylbut-2-enyl
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isoprenoids
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mecpp
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mevalonate
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plastids
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prosthetic
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epr
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paramagnetic
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iron-sulfur
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isopentenyl
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dimethylallyl
- 1.17.7.3
-
e-4-hydroxy-3-methylbut-2-enyl
-
isoprenoids
-
mecpp
- mevalonate
-
plastids
-
prosthetic
- epr
-
paramagnetic
-
iron-sulfur
-
isopentenyl
-
dimethylallyl
Reaction
Synonyms
(E)-4-hydroxy-3-methylbut-2-en-1-yl-diphosphate:protein-disulfide oxidoreductase (hydrating), (E)-4-hydroxy-3-methylbut-2-enyl diphosphate synthase, 2-C-methyl-D-erythritol-2,4-cyclodiphosphate reductase, 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase, GcpE, HMBPP synthase, IspG
ECTree
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Purification
Purification on EC 1.17.7.3 - (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase (flavodoxin)
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His6-tagged enzyme. The purified protein is inactive. It is activated after reconstitution of its Fe/S cluster
recombinant fusion protein comprising maltose binding protein and IspG protein domains
the recombinant MalE/IspG fusion protein (expression level of about 40% with respect to the total soluble cell protein) is purified by affinity chromatography on an amylose resin FF column that is developed with a gradient of 0-10 mM maltose under anaerobic conditions, whereby the enzyme is enriched by a factor of 2.4