1.1.1.6: glycerol dehydrogenase This is an abbreviated version! For detailed information about glycerol dehydrogenase, go to the full flat file .
Reaction
glycerol +
NAD+ =
Glycerone +
NADH +
H+
Synonyms B4100_2156, CglD, dehydrogenase, glycerol, GDH, GDH2, GLD, Gld3, GldA, GLDH , GlhA, glycerin dehydrogenase, glycerol dehydrogenase, glycerol NAD 2-oxidoreductase, glycerol:NAD+ 2-oxidoreductase, GlyDH, Gro dehydrogenase, GroDHase, GSH, gycerol dehydrogenase, NAD+-dependent glycerol dehydrogenase, NAD-linked glycerol dehydrogenase, NAD-specific glycerol dehydrogenase, TmGlyDH, TTHADH
ECTree
pH Optimum
pH Optimum on EC 1.1.1.6 - glycerol dehydrogenase
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10 - 11
the immobilized GDH changes the optimal pH from pH 11 to 10 and exhibits high activity in a relatively wide range of pH from pH 7 to 12, while free GDH only achieves high activity at pH 11
10.5
-
in reverse micelles
10.5 - 11
-
NADH production
11 - 12
-
oxidation of glycerol
5 - 6
-
reduction of dihydroxyacetone
7.2
-
oxidation of glycerol, wild-type enzyme
7.5 - 8.5
-
reduction of dihydroxyacetone, phosphate buffer
8 - 8.5
-
oxidation of 1,2-propanediol
8.3
-
reduction of dihydroxyacetone, mutant D
9.5 - 10.5
-
oxidation of glycerol, diphosphate buffer
additional information
kinetic study of the metal ion-chelated polyethyleneimines-immobilized enzyme
10
-
-
10
-
oxidation of glycerol
10
maximum activity at pH 8.6 in CHES buffer and pH 10.0 in CAPS buffer
10
the enzyme has two pH optima at pH 8.6 in CHES buffer (78% activity) and 10.0 in CAPS buffer (100% activity)
11
-
glycerol oxidation reaction
11
recombinant His-tagged chimeric enzyme GDH-NOX
12
-
5
-
reduction of hydroxyacetone
5
mutant F245S, reduction of pyruvate
6
-
reduction of dihydroxyacetone or 3-hydroxybutane-2-one
6
-
reaction of dihydroxyacetone
7.5
assay at
7.5
-
oxidation of glycerol, mutant D
8
-
assay at
8
-
dihydroxyaceton reduction reaction
8
-
oxidation of glycerol, tetraethyl ammonium chloride/tetraethylammonium hydroxide buffer
8.6
-
reduction of dihydroxyacetone, wild-type enzyme
8.6
maximum activity at pH 8.6 in CHES buffer and pH 10.0 in CAPS buffer
8.6
the enzyme has two pH optima at pH 8.6 in CHES buffer (78% activity) and 10.0 in CAPS buffer (100% activity)
9
-
oxidation of glycerol
9
-
oxidation of glycerol or butanediol
9
-
oxidation of glycerol
9.5
-
in an aqueous buffer system