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Literature summary for 1.1.1.6 extracted from
- Codon-optimized NADH oxidase gene expression and gene fusion with glycerol dehydrogenase for bienzyme system with cofactor regeneration (2015), PLoS ONE, 10, e0128412.
Application
| Application | Comment | Organism |
|---|---|---|
| analysis | the recombinant chimeric fusion enzyme GDH-NOX has a potential application for quick glycerol analysis and dioxyacetone biosynthesis | Klebsiella pneumoniae |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| recombinant expression of the His-tagged enzyme encoding gene fused to the codon-optimized NADH oxidase gene nox from Lactobacillus brevis, chimeric enzyme GDH-NOX, in Escherichia coli strain BL21(DE3) resulting in bioconversion of glycerol to dihydroxyacetone in the cells | Klebsiella pneumoniae |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | the glycerol dehydrogenase gene from Klebsiella pneumoniae is fused to codon-optimized NADH oxidase gene from Lactobacillus brevis. Gene fusion of glycerol dehydrogenase (GDH) and NOX forms a bifunctional multienzyme for bioconversion of glycerol coupled with coenzyme regeneration | Klebsiella pneumoniae |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0513 | - |
NADH | recombinant chimeric enzyme GDH-NOX, pH 11.0, 37°C | Klebsiella pneumoniae |  |
| 19.4 | - |
glycerol | recombinant chimeric enzyme GDH-NOX, pH 11.0, 37°C | Klebsiella pneumoniae | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glycerol + NAD+ | Klebsiella pneumoniae | - |
glycerone + NADH + H+ | - |
r | |
| glycerol + NAD+ | Klebsiella pneumoniae DSM 2026 | - |
glycerone + NADH + H+ | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Klebsiella pneumoniae | B2ZPN8 | - |
- |
| Klebsiella pneumoniae DSM 2026 | B2ZPN8 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged chimeric enzyme GDH-NOX from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Klebsiella pneumoniae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 15.7 | - |
recombinant His-tagged chimeric enzyme GDH-NOX, pH 11.0, 37°C | Klebsiella pneumoniae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glycerol + NAD+ | - |
Klebsiella pneumoniae | glycerone + NADH + H+ | - |
r | |
| glycerol + NAD+ | - |
Klebsiella pneumoniae DSM 2026 | glycerone + NADH + H+ | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GDH | - |
Klebsiella pneumoniae |
| GldA | - |
Klebsiella pneumoniae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 11 | - |
recombinant His-tagged chimeric enzyme GDH-NOX | Klebsiella pneumoniae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 11 | - |
recombinant His-tagged chimeric enzyme GDH-NOX | Klebsiella pneumoniae |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Klebsiella pneumoniae | |
| NADH | - |
Klebsiella pneumoniae | |
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