1.1.1.6: glycerol dehydrogenase
This is an abbreviated version!
For detailed information about glycerol dehydrogenase, go to the full flat file.
Reaction
Synonyms
B4100_2156, CglD, dehydrogenase, glycerol, GDH, GDH2, GLD, Gld3, GldA, GLDH
ECTree
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Purification
Purification on EC 1.1.1.6 - glycerol dehydrogenase
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gene gldA, recombinant C-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, ultrafiltration, and dialysis
HisTrap column chromatography and Superdex 200 gel filtration
immobilized metal ion affinity chromatography (Co2+)
immobilized metal ion affinity chromatography (Ni2+)
purified with 25fold purification and 68% yield using a single chromatographic step
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purity estimated from SDS-PAGE: 95%
recombinant His-tagged chimeric enzyme GDH-NOX from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant His-tagged enzyme from Escherichia coli strain BL21 (DE3) by immobilized metal affinity chromatography or by heat shock treatment, both resulting in about the same protein purity of over 90%
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recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
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recombinant His-tagged enzyme from strain BL21(DE3) by nickel affinity chromatography
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recombinant N-terminally His6-tagged enzyme from Escherichia coli train BL21(DE3)pLysS by nicke affinity chromatography and gel filtration
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recombinant protein, purified to homogeneity using a combination of heat-shock and chromatographic methods
recombinent His-tagged enzyme from Escherichia coli strain BL21(DE3) pET-32a by nickel affinity chromatography, desalting gel filtration, and ultrafiltration