EC Number   |
Protein Variants |
Reference |
|---|
   3.1.1.75 | A134G |
PHB depolymerase activity similar to wild type |
664977 |
| 3.1.1.75 | A134G, H135L |
PHB depolymerase activity similar to wild type, 2fold lipase activity compared to wild type |
664977 |
| 3.1.1.75 | A66V |
random mutagenesis, the mutant enzymes shows increased activity with 4-nitrophenyl butyrate as compared to the wild-type enzyme |
-, 729068 |
| 3.1.1.75 | A66V/N251S/T290A |
random mutagenesis, the mutant enzymes shows altered activity with 4-nitrophenyl butyrate as compared to the wild-type enzyme |
729068 |
| 3.1.1.75 | A66V/N285D/G310G |
random mutagenesis, the mutant enzymes shows altered activity with 4-nitrophenyl butyrate as compared to the wild-type enzyme |
729068 |
| 3.1.1.75 | A66V/T145S/N285D |
random mutagenesis, the mutant enzymes shows altered activity with 4-nitrophenyl butyrate as compared to the wild-type enzyme |
729068 |
| 3.1.1.75 | C178A |
no degradation of amorphous poly(3-hydroxybutyrate) granules or 3-hydroxybutyrate oligomers |
664693 |
| 3.1.1.75 | D209N |
site-directed mutagenesis, inactive mutant |
-, 730744 |
| 3.1.1.75 | D242A |
no PHB depolymerase activity |
664977 |
| 3.1.1.75 | D242N |
no PHB depolymerase activity |
664977 |
