EC Number |
Protein Variants |
Reference |
---|
3.4.22.10 | C192S |
no autocatalytic processing of the 40000 Da zymogen to the 28000 Da mature form, no proteolytic activity |
81463 |
3.4.22.10 | C192S |
inactive mutant |
-, 667311, 667426, 668911, 670124 |
3.4.22.10 | C47S |
crystal structure analysis |
668772 |
3.4.22.10 | more |
construction of an isogenic inactive mutant strain M6 |
670124 |
3.4.22.10 | C47S |
site-directed mutagenesis, inactive mutant |
-, 670312 |
3.4.22.10 | C192S |
lacks protease activity |
680145 |
3.4.22.10 | C192S |
inactive |
680146, 681702 |
3.4.22.10 | G308S |
behaviour is similar to the wild type enzyme |
680146 |
3.4.22.10 | C47S |
inactive mutant |
698880 |
3.4.22.10 | D9N |
protease activity (microgram/min/mg) substrate azocasein: 256, substrate proSPE B C47S mutant: 250, compared to wild-type 341 and 378, respectively |
698880 |