3.4.22.10 C192S no autocatalytic processing of the 40000 Da zymogen to the 28000 Da mature form, no proteolytic activity 81463 3.4.22.10 C192S inactive mutant -, 667311, 667426, 668911, 670124 3.4.22.10 C47S crystal structure analysis 668772 3.4.22.10 additional information construction of an isogenic inactive mutant strain M6 670124 3.4.22.10 C47S site-directed mutagenesis, inactive mutant -, 670312 3.4.22.10 C192S lacks protease activity 680145 3.4.22.10 C192S inactive 680146, 681702 3.4.22.10 G308S behaviour is similar to the wild type enzyme 680146 3.4.22.10 C47S inactive mutant 698880 3.4.22.10 D9N protease activity (microgram/min/mg) substrate azocasein: 256, substrate proSPE B C47S mutant: 250, compared to wild-type 341 and 378, respectively 698880