EC Number   |
Protein Variants |
Reference |
|---|
   3.5.4.33 | E92A |
mutation in subunit ADAT2, inactive mutant enzyme |
721030 |
| 3.5.4.33 | E92A/K216A/R217A/K218A/R219A |
inactive mutant enzyme |
721030 |
| 3.5.4.33 | F144A |
mutations results in a reduction in enzymatic activity of about 800-fold |
718839 |
| 3.5.4.33 | F145A |
inactive mutant enzyme |
718839 |
| 3.5.4.33 | H252A |
inactive mutant enzyme, mutation in subunit ADAT3 |
719934 |
| 3.5.4.33 | H90A |
inactive mutant enzyme, mutation in subunit ADAT2 |
719934 |
| 3.5.4.33 | K216A |
mutation in subunit ADAT2, kcat/Km is 12% compared to the wild-type value |
721030 |
| 3.5.4.33 | K218A |
mutation in subunit ADAT2, kcat/Km is 24% compared to the wild-type value |
721030 |
| 3.5.4.33 | more |
deletion of the last 10 amino acids at the C terminus of TbADAT2 abolishes tRNA binding. In addition, single alanine replacements of a string of positively charged amino acids (KRKRK) lead to binding defects that correlate with losses in enzyme activity |
721030 |
| 3.5.4.33 | R149A |
mutations results in a reduction in enzymatic activity of about 800-fold |
718839 |
