BRENDA - Enzyme Database show
show all sequences of 3.5.4.33

Biochemical and structural studies of A-to-I editing by tRNA:A34 deaminases at the wobble position of transfer RNA

Elias, Y.; Huang, R.H.; Biochemistry 44, 12057-12065 (2005)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
overexpression in Escherichi coli
Aquifex aeolicus
overexpression in Escherichi coli
Escherichia coli
overexpression in Escherichia coli
Agrobacterium tumefaciens
overexpression in Escherichia coli
Saccharomyces cerevisiae
Crystallization (Commentary)
Crystallization
Organism
tadA missing the last eight amino acids at the C-terminus is produced high-quality crystals, and the structure is determined at 1.6 A resolution, hanging drop vapor diffusion method
Agrobacterium tumefaciens
Engineering
Amino acid exchange
Commentary
Organism
F144A
mutations results in a reduction in enzymatic activity of about 800-fold
Agrobacterium tumefaciens
F145A
inactive mutant enzyme
Agrobacterium tumefaciens
R149A
mutations results in a reduction in enzymatic activity of about 800-fold
Agrobacterium tumefaciens
R94A
mutation substantially 220fold reduces the enzymatic activity
Agrobacterium tumefaciens
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Zn2+
the active site contains a zinc ion, which is coordinated by three strictly conserved amino acids, His53, Cys83, and Cys86
Agrobacterium tumefaciens
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
adenine34 in tRNAArg + H2O
Escherichia coli
-
hypoxanthine34 in tRNAArg + NH3
-
-
?
adenine34 in tRNAArg + H2O
Aquifex aeolicus
-
hypoxanthine34 in tRNAArg + NH3
-
-
?
adenine34 in tRNAArg + H2O
Agrobacterium tumefaciens
-
hypoxanthine34 in tRNAArg + NH3
-
-
?
adenine34 in tRNAArg + H2O
Saccharomyces cerevisiae
-
hypoxanthine34 in tRNAArg + NH3
-
-
?
adenine34 in tRNAArg + H2O
Saccharomyces cerevisiae ATCC 204508
-
hypoxanthine34 in tRNAArg + NH3
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Agrobacterium tumefaciens
A9CK16
-
-
Aquifex aeolicus
-
-
-
Escherichia coli
-
-
-
Saccharomyces cerevisiae
P47058 and Q9URQ3
P47058: TAD2 and Q9URQ3: TAD3
-
Saccharomyces cerevisiae ATCC 204508
P47058 and Q9URQ3
P47058: TAD2 and Q9URQ3: TAD3
-
Purification (Commentary)
Commentary
Organism
-
Agrobacterium tumefaciens
-
Aquifex aeolicus
-
Saccharomyces cerevisiae
-
Escherichia coli
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
adenine34 in tRNAArg + H2O
-
718839
Escherichia coli
hypoxanthine34 in tRNAArg + NH3
-
-
-
?
adenine34 in tRNAArg + H2O
-
718839
Aquifex aeolicus
hypoxanthine34 in tRNAArg + NH3
-
-
-
?
adenine34 in tRNAArg + H2O
-
718839
Agrobacterium tumefaciens
hypoxanthine34 in tRNAArg + NH3
-
-
-
?
adenine34 in tRNAArg + H2O
-
718839
Saccharomyces cerevisiae
hypoxanthine34 in tRNAArg + NH3
-
-
-
?
adenine34 in tRNAArg + H2O
Escherichia coli tRNAArg2. A critical role in tRNA substrate recognition is proposed for the conserved FFxxxR motif at the C-terminus (unique to tadA)
718839
Escherichia coli
hypoxanthine34 in tRNAArg + NH3
-
-
-
?
adenine34 in tRNAArg + H2O
Escherichia coli tRNAArg2. A critical role in tRNA substrate recognition is proposed for the conserved FFxxxR motif at the C-terminus (unique to tadA)
718839
Aquifex aeolicus
hypoxanthine34 in tRNAArg + NH3
-
-
-
?
adenine34 in tRNAArg + H2O
Escherichia coli tRNAArg2. A critical role in tRNA substrate recognition is proposed for the conserved FFxxxR motif at the C-terminus (unique to tadA)
718839
Agrobacterium tumefaciens
hypoxanthine34 in tRNAArg + NH3
-
-
-
?
adenine34 in tRNAArg + H2O
Escherichia coli tRNAArg2. A critical role in tRNA substrate recognition is proposed for the conserved FFxxxR motif at the C-terminus (unique to tadA)
718839
Saccharomyces cerevisiae
hypoxanthine34 in tRNAArg + NH3
-
-
-
?
adenine34 in tRNAArg + H2O
-
718839
Saccharomyces cerevisiae ATCC 204508
hypoxanthine34 in tRNAArg + NH3
-
-
-
?
adenine34 in tRNAArg + H2O
Escherichia coli tRNAArg2. A critical role in tRNA substrate recognition is proposed for the conserved FFxxxR motif at the C-terminus (unique to tadA)
718839
Saccharomyces cerevisiae ATCC 204508
hypoxanthine34 in tRNAArg + NH3
-
-
-
?
Subunits
Subunits
Commentary
Organism
homodimer
-
Agrobacterium tumefaciens
homodimer
-
Aquifex aeolicus
homodimer
-
Escherichia coli
homodimer
-
Saccharomyces cerevisiae
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
37
-
assay at
Agrobacterium tumefaciens
37
-
assay at
Aquifex aeolicus
37
-
assay at
Escherichia coli
37
-
assay at
Saccharomyces cerevisiae
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
-
assay at
Agrobacterium tumefaciens
6
-
assay at
Aquifex aeolicus
6
-
assay at
Escherichia coli
6
-
assay at
Saccharomyces cerevisiae
Cloned(Commentary) (protein specific)
Commentary
Organism
overexpression in Escherichi coli
Aquifex aeolicus
overexpression in Escherichi coli
Escherichia coli
overexpression in Escherichia coli
Agrobacterium tumefaciens
overexpression in Escherichia coli
Saccharomyces cerevisiae
Crystallization (Commentary) (protein specific)
Crystallization
Organism
tadA missing the last eight amino acids at the C-terminus is produced high-quality crystals, and the structure is determined at 1.6 A resolution, hanging drop vapor diffusion method
Agrobacterium tumefaciens
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
F144A
mutations results in a reduction in enzymatic activity of about 800-fold
Agrobacterium tumefaciens
F145A
inactive mutant enzyme
Agrobacterium tumefaciens
R149A
mutations results in a reduction in enzymatic activity of about 800-fold
Agrobacterium tumefaciens
R94A
mutation substantially 220fold reduces the enzymatic activity
Agrobacterium tumefaciens
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Zn2+
the active site contains a zinc ion, which is coordinated by three strictly conserved amino acids, His53, Cys83, and Cys86
Agrobacterium tumefaciens
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
adenine34 in tRNAArg + H2O
Escherichia coli
-
hypoxanthine34 in tRNAArg + NH3
-
-
?
adenine34 in tRNAArg + H2O
Aquifex aeolicus
-
hypoxanthine34 in tRNAArg + NH3
-
-
?
adenine34 in tRNAArg + H2O
Agrobacterium tumefaciens
-
hypoxanthine34 in tRNAArg + NH3
-
-
?
adenine34 in tRNAArg + H2O
Saccharomyces cerevisiae
-
hypoxanthine34 in tRNAArg + NH3
-
-
?
adenine34 in tRNAArg + H2O
Saccharomyces cerevisiae ATCC 204508
-
hypoxanthine34 in tRNAArg + NH3
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Agrobacterium tumefaciens
-
Aquifex aeolicus
-
Escherichia coli
-
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
adenine34 in tRNAArg + H2O
-
718839
Escherichia coli
hypoxanthine34 in tRNAArg + NH3
-
-
-
?
adenine34 in tRNAArg + H2O
-
718839
Aquifex aeolicus
hypoxanthine34 in tRNAArg + NH3
-
-
-
?
adenine34 in tRNAArg + H2O
-
718839
Agrobacterium tumefaciens
hypoxanthine34 in tRNAArg + NH3
-
-
-
?
adenine34 in tRNAArg + H2O
-
718839
Saccharomyces cerevisiae
hypoxanthine34 in tRNAArg + NH3
-
-
-
?
adenine34 in tRNAArg + H2O
Escherichia coli tRNAArg2. A critical role in tRNA substrate recognition is proposed for the conserved FFxxxR motif at the C-terminus (unique to tadA)
718839
Escherichia coli
hypoxanthine34 in tRNAArg + NH3
-
-
-
?
adenine34 in tRNAArg + H2O
Escherichia coli tRNAArg2. A critical role in tRNA substrate recognition is proposed for the conserved FFxxxR motif at the C-terminus (unique to tadA)
718839
Aquifex aeolicus
hypoxanthine34 in tRNAArg + NH3
-
-
-
?
adenine34 in tRNAArg + H2O
Escherichia coli tRNAArg2. A critical role in tRNA substrate recognition is proposed for the conserved FFxxxR motif at the C-terminus (unique to tadA)
718839
Agrobacterium tumefaciens
hypoxanthine34 in tRNAArg + NH3
-
-
-
?
adenine34 in tRNAArg + H2O
Escherichia coli tRNAArg2. A critical role in tRNA substrate recognition is proposed for the conserved FFxxxR motif at the C-terminus (unique to tadA)
718839
Saccharomyces cerevisiae
hypoxanthine34 in tRNAArg + NH3
-
-
-
?
adenine34 in tRNAArg + H2O
-
718839
Saccharomyces cerevisiae ATCC 204508
hypoxanthine34 in tRNAArg + NH3
-
-
-
?
adenine34 in tRNAArg + H2O
Escherichia coli tRNAArg2. A critical role in tRNA substrate recognition is proposed for the conserved FFxxxR motif at the C-terminus (unique to tadA)
718839
Saccharomyces cerevisiae ATCC 204508
hypoxanthine34 in tRNAArg + NH3
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
homodimer
-
Agrobacterium tumefaciens
homodimer
-
Aquifex aeolicus
homodimer
-
Escherichia coli
homodimer
-
Saccharomyces cerevisiae
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
37
-
assay at
Agrobacterium tumefaciens
37
-
assay at
Aquifex aeolicus
37
-
assay at
Escherichia coli
37
-
assay at
Saccharomyces cerevisiae
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
-
assay at
Agrobacterium tumefaciens
6
-
assay at
Aquifex aeolicus
6
-
assay at
Escherichia coli
6
-
assay at
Saccharomyces cerevisiae
Other publictions for EC 3.5.4.33
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
718952
Su
A-to-I and C-to-U editing with ...
Agrobacterium tumefaciens, Aquifex aeolicus, Escherichia coli, Streptococcus pyogenes
Biochemistry
76
932-937
2011
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4
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9
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4
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4
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4
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719934
Spears
A single zinc ion is sufficien ...
Trypanosoma brucei
J. Biol. Chem.
286
20366-20374
2011
-
-
-
-
11
-
-
4
-
1
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1
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4
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1
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1
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3
1
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11
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4
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1
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1
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1
-
1
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-
3
1
-
-
-
-
-
-
-
4
4
721030
Ragone
The C-terminal end of the Tryp ...
Trypanosoma brucei
RNA
17
1296-1306
2011
-
-
1
-
9
-
-
6
-
-
-
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-
1
-
-
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1
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1
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6
1
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1
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9
-
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6
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-
1
-
1
-
-
6
1
-
-
-
-
-
-
-
6
6
713269
Delannoy
Arabidopsis tRNA adenosine dea ...
Arabidopsis thaliana
Plant Cell
21
2058-2071
2009
-
-
1
-
-
-
-
-
2
-
-
1
-
7
-
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2
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1
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2
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1
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2
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1
1
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-
721023
Karcher
Identification of the chloropl ...
Arabidopsis thaliana
RNA
15
1251-1257
2009
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1
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1
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1
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6
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1
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1
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1
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1
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1
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1
1
-
-
-
698470
Luo
Transition state structure of ...
Escherichia coli
J. Am. Chem. Soc.
130
2649-2655
2008
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1
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1
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1
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719843
Tsutsumi
Wobble inosine tRNA modificati ...
Schizosaccharomyces pombe
J. Biol. Chem.
282
33459-33465
2007
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-
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4
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1
1
-
-
-
720892
Rubio
An adenosine-to-inosine tRNA-e ...
Trypanosoma brucei
Proc. Natl. Acad. Sci. USA
104
7821-7826
2007
-
-
-
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1
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4
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2
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721010
Lee
Crystal structure of the tRNA- ...
Streptococcus pyogenes
Proteins
68
1016-1019
2007
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1
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689140
Losey
Crystal structure of Staphyloc ...
Staphylococcus aureus
Nat. Struct. Mol. Biol.
13
153-159
2006
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1
1
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1
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669323
Kuratani
Crystal structure of tRNA aden ...
Aquifex aeolicus
J. Biol. Chem.
280
16002-16008
2005
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1
1
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1
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1
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1
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1
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2
1
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1
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1
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1
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718485
Ku
Crystallization and preliminar ...
Streptococcus pyogenes, Streptococcus pyogenes ATCC BAA-946
Acta Crystallogr. Sect. F
61
375-357
2005
-
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1
1
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5
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1
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1
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1
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718839
Elias
Biochemical and structural stu ...
Agrobacterium tumefaciens, Aquifex aeolicus, Escherichia coli, Saccharomyces cerevisiae, Saccharomyces cerevisiae ATCC 204508
Biochemistry
44
12057-12065
2005
-
-
4
1
4
-
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1
-
5
-
14
-
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4
-
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10
4
4
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4
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4
-
1
4
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1
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5
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4
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10
4
4
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4
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-
719356
Wolf
tadA, an essential tRNA-specif ...
Escherichia coli
EMBO J.
21
3841-3851
2002
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1
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1
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1
1
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4
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1
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2
1
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1
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1
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1
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An adenosine deaminase that ge ...
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Mechanism, specificity and gen ...
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