Cloned (Comment) | Organism |
---|---|
- |
Trypanosoma brucei |
Protein Variants | Comment | Organism |
---|---|---|
C-terDELTA10 | mutation in subunit ADAT2, inactive mutant enzyme | Trypanosoma brucei |
C-terDELTA5 | mutation in subunit ADAT2, kcat/Km is 72% compared to the wild-type value | Trypanosoma brucei |
E92A | mutation in subunit ADAT2, inactive mutant enzyme | Trypanosoma brucei |
E92A/K216A/R217A/K218A/R219A | inactive mutant enzyme | Trypanosoma brucei |
K216A | mutation in subunit ADAT2, kcat/Km is 12% compared to the wild-type value | Trypanosoma brucei |
K218A | mutation in subunit ADAT2, kcat/Km is 24% compared to the wild-type value | Trypanosoma brucei |
additional information | deletion of the last 10 amino acids at the C terminus of TbADAT2 abolishes tRNA binding. In addition, single alanine replacements of a string of positively charged amino acids (KRKRK) lead to binding defects that correlate with losses in enzyme activity | Trypanosoma brucei |
R217A | mutation in subunit ADAT2, kcat/Km is 16% compared to the wild-type value | Trypanosoma brucei |
R219A | mutation in subunit ADAT2, kcat/Km is 20% compared to the wild-type value | Trypanosoma brucei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00075 | - |
adenine34 in tRNAVal | 27°C, pH 8.0, wild-type enzyme | Trypanosoma brucei | |
0.00076 | - |
adenine34 in tRNAVal | 27°C, pH 8.0, mutant enzyme C-terDELTA5, mutation in subunit ADAT2 | Trypanosoma brucei | |
0.002 | - |
adenine34 in tRNAVal | 27°C, pH 8.0, mutant enzyme R218A | Trypanosoma brucei | |
0.0025 | - |
adenine34 in tRNAVal | 27°C, pH 8.0, mutant enzyme R217A | Trypanosoma brucei | |
0.0028 | - |
adenine34 in tRNAVal | 27°C, pH 8.0, mutant enzyme R219A | Trypanosoma brucei | |
0.003 | - |
adenine34 in tRNAVal | 27°C, pH 8.0, mutant enzyme K216A | Trypanosoma brucei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma brucei | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
adenine34 in tRNAVal + H2O | - |
Trypanosoma brucei | hypoxanthine34 in tRNAVal + NH3 | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
27 | - |
assay at | Trypanosoma brucei |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0015 | - |
adenine34 in tRNAVal | 27°C, pH 8.0, mutant enzyme R217A | Trypanosoma brucei | |
0.0017 | - |
adenine34 in tRNAVal | 27°C, pH 8.0, mutant enzyme K216A | Trypanosoma brucei | |
0.002 | - |
adenine34 in tRNAVal | 27°C, pH 8.0, mutant enzyme R218A | Trypanosoma brucei | |
0.0022 | - |
adenine34 in tRNAVal | 27°C, pH 8.0, mutant enzyme R219A | Trypanosoma brucei | |
0.0023 | - |
adenine34 in tRNAVal | 27°C, pH 8.0, mutant enzyme C-terDELTA5, mutation in subunit ADAT2 | Trypanosoma brucei | |
0.0032 | - |
adenine34 in tRNAVal | 27°C, pH 8.0, wild-type enzyme | Trypanosoma brucei |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Trypanosoma brucei |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.57 | - |
adenine34 in tRNAVal | 27°C, pH 8.0, mutant enzyme K216A | Trypanosoma brucei | |
0.59 | - |
adenine34 in tRNAVal | 27°C, pH 8.0, mutant enzyme R217A | Trypanosoma brucei | |
0.79 | - |
adenine34 in tRNAVal | 27°C, pH 8.0, mutant enzyme R219A | Trypanosoma brucei | |
1 | - |
adenine34 in tRNAVal | 27°C, pH 8.0, mutant enzyme R218A | Trypanosoma brucei | |
3.03 | - |
adenine34 in tRNAVal | 27°C, pH 8.0, mutant enzyme C-terDELTA5, mutation in subunit ADAT2 | Trypanosoma brucei | |
4.27 | - |
adenine34 in tRNAVal | 27°C, pH 8.0, wild-type enzyme | Trypanosoma brucei |