EC Number   |
Reference |
|---|
   3.1.1.6 | - |
23605, 35234, 35238 |
| 3.1.1.6 | hanging drop vapor diffusion method, using 170 mM ammonium sulfate, 28% (w/v) PEG 4000, 5% (v/v) glycerol and 0.5% (w/v) n-octyl-beta-D-glucopyranoside |
750537 |
| 3.1.1.6 | hanging drop vapor diffusion method, using 20% (w/v) PEG-3000, 0.1 M HEPES pH 7.5, 0.2 M NaCl (selenomethionine-substituted enzyme) or nanodrop vapor diffusion method, using 0.2 M calcium acetate hydrate, 20% (w/v) PEG 3350, pH 7.3 at 20°C (native enzyme) |
730907 |
| 3.1.1.6 | His-tagged protein, both in the native form and with selenomethionine substitution |
663520 |
| 3.1.1.6 | molecular dynamic simulations and automated docking with R and S enantiomers of substrate 1,1,1-trifluoro-2-phenyl-but-3-yn-1-yl acetate using structure of wild-type and structural model of double mutant E188W/M193C, S enantiomer fits better in active site of mutant E188W/M193C because subtrates phenyl group points out of it, docking of the preferred R enantiomer by the wild-type with lower free energy than docking of the S enantiomer |
695582 |
| 3.1.1.6 | purified selenomethionine enzyme, 6-7 mg/ml, pure or complexed with the inhibitor dimethylarsinic acid, crystal growth in 1.8 M ammonium sulfate, 0.1 M NaCl, 0.1 M cacodylate, pH 6.5, for crystallization of native methionine enzyme 1.7 M ammonium sulfate, 0.1 M NaCl, 0.1 M BES, pH 6.4, is used, X-ray structure determination and analysis at 1.3 A and 1.45 A resolution |
652394 |
| 3.1.1.6 | S10A mutant enzyme complexed with an acetate ion, sitting drop vapor diffusion method, using 0.1 M MES buffer (pH 6.5), 15-20% (v/v) PEGME 550, and 10 mM ZnSO4 |
749806 |
| 3.1.1.6 | structural modelling using Cn3D 4.1 software and plant strictosidine synthase as model structure, six-bladed beta-propeller structure |
696095 |
| 3.1.1.6 | structural superimposition of homology-generated model with Alicyclobacillus acidocaldarius EST2 and Escherichia coli beta-cystathionase MalY revealed nine amino acid consensus sequence putatively involved in protein-protein interactions, amino acids 178-184 are putative core of interaction with MalY and MalT |
701087 |
| 3.1.1.6 | using 0.2 M ammonium phosphate (pH 4.6), and 20% (w/v) polyethylene glycol 3350 |
750541 |
