EC Number |
pH Stability |
pH Stability Maximum |
Reference |
---|
1.14.18.1 | -999 |
- |
highest activity and stability within a neutral and alkaline pH range |
673560 |
1.14.18.1 | -999 |
- |
PPO activity, as a function of pH, is determined under standard conditions using various buffers in the pH buffering range 2.0-12.0 |
686845 |
1.14.18.1 | 2 |
10 |
PPO exhibits a gradual increase in activity from pH 2.0-4.5, PPO exhibits a gradual decline in activity at pH above 4.5 with a slight recovery at pH 10.0 |
698434 |
1.14.18.1 | 2.5 |
3.5 |
the enzyme is most unstable at pH 2.5-3.5 retaining less than 10% of original activity after 2 h |
712619 |
1.14.18.1 | 3 |
- |
4°C, 24 h, more than 80% loss of activity |
636371 |
1.14.18.1 | 3 |
- |
purified native enzyme, loss of 50% activity |
745110 |
1.14.18.1 | 3 |
8 |
isozyme PPO III, 90% activity after 18 h at pH 3.0 |
636356 |
1.14.18.1 | 3 |
9 |
the enzyme is extremely stable between pH 3.0 and 9.0 after 24 h incubation at 4°C retaining at least 85% of its original activity |
712053 |
1.14.18.1 | 3 |
10 |
partially purified enzyme, stable, 90% of maximal activity within this range |
675164 |
1.14.18.1 | 3 |
10 |
stable between pH 4.0 and 10.0 retaining more than 80% of its activity in the pH range of 5.0-9.0, PPO is rapidly inactivated by the pH below 5.0, the activity of recombinant PPO gradually increases with pH value in the range of 5-7, the activity decreases rapidly below and above pH 7.5, more than 70% of the optimal activity is kept between pH 7.5 and 10.0, after incubating at pH 11.0 for 1 h the recombinant PPO still retains 74% of its activity whereas at pH 3.0 it is left with only 39.4% |
698038 |