Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ascorbic acid | - |
Neoboletus erythropus | |
benzoic acid | - |
Neoboletus erythropus | |
Ca2+ | 74.2% residual activity at 1 mM | Neoboletus erythropus | |
Cu2+ | 88.3% residual activity at 1 mM | Neoboletus erythropus | |
Li+ | 72.5% residual activity at 1 mM | Neoboletus erythropus | |
Mn2+ | 62.9% residual activity at 1 mM | Neoboletus erythropus | |
Ni2+ | 56.6% residual activity at 1 mM | Neoboletus erythropus | |
Sodium azide | - |
Neoboletus erythropus | |
Sodium metabisulfite | - |
Neoboletus erythropus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.8 | - |
4-methylcatechol | pH 8.0, 20°C | Neoboletus erythropus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Al3+ | 127.3% activity at 1 mM | Neoboletus erythropus | |
additional information | the enzyme is not influenced by Zn2+, Cr2+, Na+, K+, Mg2+, and Cd2+ | Neoboletus erythropus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
SDS-PAGE | Neoboletus erythropus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Neoboletus erythropus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Sepharose 4B-L-tyrosine-p-aminobenzoic acid affinity column chromatography, gel filtration | Neoboletus erythropus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylcatechol + O2 | highest activity | Neoboletus erythropus | 4-methyl-1,2-benzoquinone + H2O | - |
? | |
L-DOPA + O2 | - |
Neoboletus erythropus | dopaquinone + H2O | - |
? | |
L-tyrosine + O2 | - |
Neoboletus erythropus | L-DOPA + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
polyphenol oxidase | - |
Neoboletus erythropus |
PPO | - |
Neoboletus erythropus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
using 4-methylcatechol as a substrate | Neoboletus erythropus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 80 | the enzyme is stable between 10 and 30°C after 24 h incubation, The enzyme activity declines as the temperature increases but the enzyme is not completely inactivated even at 80°C | Neoboletus erythropus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
using 4-methylcatechol as a substrate | Neoboletus erythropus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | 9 | the enzyme is extremely stable between pH 3.0 and 9.0 after 24 h incubation at 4°C retaining at least 85% of its original activity | Neoboletus erythropus |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.07 | - |
pH 8.0, 20°C | Neoboletus erythropus | Sodium metabisulfite | |
0.08 | - |
pH 8.0, 20°C | Neoboletus erythropus | ascorbic acid | |
10 | - |
pH 8.0, 20°C | Neoboletus erythropus | Sodium azide | |
10.2 | - |
pH 8.0, 20°C | Neoboletus erythropus | benzoic acid |