EC Number |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Reference |
---|
3.4.21.83 | 30 |
- |
30 min, 25% loss of activity |
731412 |
3.4.21.83 | 30 |
- |
rapid loss of activity above |
717235 |
3.4.21.83 | 37 |
- |
fluorescence intensity of enzyme decreases with increasing heat treatment time and the maximum fluorescence wavelength shifts slightly towards longer wavelength, 340-345 nm and 342-346 nm. The fluorescence intensity in 50% glycerol does not change significantly following heat treatment. The maximum fluorescence wavelength also shifts slightly, 337-340 nm and 339-340 nm |
732874 |
3.4.21.83 | 37 |
- |
wild-type and mutant PSP enzymes can undergo reversible thermal inactivation at 37°C, pH 8.0, with activity being restored or even increased with respect to the initial one upon subsequent cooling. The process can be repeated several times, with the same results achieved (up to 5 cycles). This effect can be explained by a shift in the equilibrium between the inactive open form of the enzyme and the active closed one upon variation of the incubation temperature, overview |
752409 |
3.4.21.83 | 40 |
- |
3 h, stable |
29785 |
3.4.21.83 | 40 |
- |
30 min, 75% loss of activiy |
731412 |
3.4.21.83 | 42 |
- |
30 min, wild-type, 89% residual activity, mutants E172Q, E490Q, E524Q and R689A 99%, 9%, 49% and 73% residual activity, respectively |
732011 |
3.4.21.83 | 42 |
- |
enzyme retains its dimeric structure and is fully active up to 42°C |
732683 |
3.4.21.83 | 45 |
- |
15 min, stable below |
670791 |
3.4.21.83 | 45 |
- |
30 min, pH 8.0, 50% loss of activity |
29784, 29786 |